化学
脂质体
膜
生物物理学
细胞
脂质双层融合
细胞膜
阳离子脂质体
生物分子
HEK 293细胞
细胞生物学
生物化学
转染
生物
基因
作者
Ágnes Csiszár,Nils Hersch,Sabine Dieluweit,Ralf Biehl,Rudolf Merkel,Bernd Hoffmann
摘要
Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was tested on single cells in culture like HEK293, myofibroblasts, cortical neurons, human macrophages, smooth muscle cells, and even on tissue. Fusogenic liposomes enabled highly efficient incorporation of molecules into mammalian cell membranes within 1 to 30 min at fully unchanged cell growth conditions and did not affect cell behavior. We hypothesize that membrane fusions were induced in all cases by the interaction of the positively charged lipids and the delocalized electron system of the aromatic group generating local dipoles and membrane instabilities. Selected applications ranging from basic research to biotechnology are envisaged here.
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