再生(生物学)
极限抗拉强度
碳二亚胺
材料科学
神经导管
生物医学工程
脚手架
电气导管
周围神经
化学
生物物理学
复合材料
解剖
高分子化学
机械工程
工程类
生物
细胞生物学
医学
作者
Li Yao,Kristen L. Billiar,Anthony J. Windebank,Abhay Pandit
出处
期刊:Tissue Engineering Part C-methods
[Mary Ann Liebert]
日期:2010-06-09
卷期号:16 (6): 1585-1596
被引量:75
标识
DOI:10.1089/ten.tec.2010.0152
摘要
In the absence of donor tissues, conduits are needed for axons to regenerate across nerve defects, yet single-channel conduits may result in axonal dispersion, and multichannel synthetic polymer conduits have failed due to dimensional instability. The goal of this study was to create a robust collagen-based nerve conduit with multiple submillimeter-diameter channels to facilitate nerve guidance. Toward this goal, we have developed a novel multistep molding technique to create single-, four-, and seven-channel conduits from collagen and examined the effects of crosslinking with 0-60 mM (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide [EDC] in N-hydroxysuccinimide) on geometric, enzymatic, and thermal stability, mechanical properties, and cellular behavior. Multichannel collagen conduits crosslinked with 30 mM EDC and 10 mM N-hydroxysuccinimide demonstrated low degradation rate (∼10% at 2 days), high shrinkage temperature (>75°C), and constant channel morphology out to 30 days in saline. Neurite outgrowth remained unaffected from cultured dorsal root ganglia explants seeded on collagen scaffolds with up to 30 mM EDC crosslinking. Compared with single-channel conduits, multichannel collagen conduits showed superior structural compressive, tensile, and bending stiffness. Taken together, these results suggest that the crosslinked multichannel collagen conduits possess favorable material and mechanical properties for nerve regeneration applications.
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