抗坏血酸
微分脉冲伏安法
检出限
化学
单层
共价键
碳二亚胺
循环伏安法
盐酸盐
自组装单层膜
电极
氧化还原
吸附
尿酸
核化学
色谱法
无机化学
电化学
有机化学
生物化学
物理化学
食品科学
作者
Reza Karimi Shervedani,Sonia Bahrani,Marzieh Samiei Foroushani,Fariborz Momenbeik
标识
DOI:10.1002/elan.201600220
摘要
Abstract A simple, stable and recoverable sensor is developed based on selective and covalent immobilization of dopamine (DA) on the gold (Au) electrode modified by mercaptopropionic acid (MPA) self‐assembled monolayers and then, activated with 1‐ethyl‐3(3‐(dimethylamino)propyl)‐carbodiimide hydrochloride (EDC)/n‐hydroxysuccinimide (NHS) activators. Fabrication steps and analytical application of this modified electrode toward quantitative determination of DA in the presence of ascorbic and uric acids are verified by cyclic and differential pulse voltammetry (CV and DPV) methods in the absence of any redox probe. The oxidation peak current of adsorbed DA changed linearly with the concentration of DA in the range of 0.5 to 600.0 μM with a detection limit of 0.064 μM, and the RSDs varied from 1.4 to 3.5 % for n=3 at each point. Finally, the fabricated sensor is used successfully for electroanalytical determination of DA in human blood plasma and pharmaceutical injection real samples.
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