斯氏假单胞菌
大肠杆菌
细菌
质粒
细菌外膜
微生物学
化学
转化(遗传学)
小泡
生物
生物化学
基因
膜
遗传学
作者
Weichuan Qiao,Wang Lian-jie,Yang Luo,Jiahui Miao
出处
期刊:Biodegradation
[Springer Nature]
日期:2021-04-22
卷期号:32 (4): 435-448
被引量:5
标识
DOI:10.1007/s10532-021-09945-y
摘要
Bacterial genetic material can be horizontally transferred between microorganisms via outer membrane vesicles (OMVs) released by bacteria. Up to now, the application of vesicle-mediated horizontal transfer of “degrading genes” in environmental remediation has not been reported. In this study, the nirS gene from an aerobic denitrification bacterium, Pseudomonas stutzeri, was enclosed in a pET28a plasmid, transformed into Escherichia coli (E. coli) DH5α and expressed in E. coli BL21. The E. coli DH5α released OMVs containing the recombination plasmid pET28a–nirS-EGFP. When compared with the free pET28a–nirS-EGFP plasmid’s inability to transform, nirS in OMVs could be transferred into E. coli BL21 with the transformation frequency of 2.76 × 106 CFU/g when the dosage of OMVs was 200 µg under natural conditions, and nirS could express successfully in recipient bacteria. Furthermore, the recipient bacteria that received OMVs containing pET28a–nirS-EGFP could produce 18.16 U/mL activity of nitrite reductase.
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