Ellagic acid-loaded, tween 80-coated, chitosan nanoparticles as a promising therapeutic approach against breast cancer: In-vitro and in-vivo study

鞣花酸 体内 药理学 壳聚糖 体外 化学 细胞毒性 药物输送 多酚 生物化学 医学 抗氧化剂 生物 生物技术 有机化学
作者
Harsheen Kaur,Sandip Ghosh,Pradeep Kumar,Biswarup Basu,Kalpana Nagpal
出处
期刊:Life Sciences [Elsevier]
卷期号:284: 119927-119927 被引量:35
标识
DOI:10.1016/j.lfs.2021.119927
摘要

Among polyphenolic phytoconstituents with anticancer properties, Ellagic acid (EA) is widely reported for its translational potential in vitro but efficient in vivo delivery of EA has been a challenge. We, for the first time, used a tween 80 coated nano delivery of Ellagic acid to evaluate its preclinical efficacy in vitro and in vivo for breast cancer. To overcome the challenges of in vivo delivery, two batches of chitosan-based nanoformulations of EA (with and without tween 80 coating) were prepared by the ionotropic gelation method. The nanoformulations were characterized and further evaluated in vitro against breast cancer cells (MCF7) and in vivo with EAC tumor-bearing mice for establishing their anticancer efficacy compared to Ellagic acid alone. A quantitative simulation study was undertaken to understand if the observed antitumor efficacy is due to the synergistic efficacy of the Chitosan-Ellagic acid combination. Results revealed that nanoformulations consist of good nano-sized encapsulation of EA and showed good drug entrapment-release capacity. Nano-encapsulated EA is biocompatible and exhibited higher cytotoxicity in vitro compared to EA alone. Similarly, significantly higher tumor regression was observed in nano-EA treated mice compared to EA alone, and best efficacy was observed with the nanoformulation with tween 80 coating. Furthermore, nanoformulations showed higher apoptosis in tumor tissues with no significant tissue toxicity in vital organs. We report synergism of Chitosan-Ellagic acid combination in the tween 80 coated nanoparticles of Ellagic acid resulting in enhanced anti-breast tumor efficacy that may be of translational value for other tumor types, too.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
高挑的果汁完成签到,获得积分10
3秒前
7秒前
英姑应助sxy采纳,获得10
8秒前
李健的小迷弟应助jack采纳,获得10
8秒前
8秒前
Yakamoz发布了新的文献求助10
9秒前
11秒前
12秒前
liuxc完成签到 ,获得积分10
12秒前
上官若男应助哈库丹采纳,获得10
12秒前
12秒前
晗月完成签到,获得积分10
13秒前
Emmmm发布了新的文献求助10
13秒前
14秒前
15秒前
Strike发布了新的文献求助10
15秒前
16秒前
欢喜的小天鹅完成签到 ,获得积分10
16秒前
czp完成签到,获得积分10
16秒前
Dmumu完成签到,获得积分10
17秒前
Akim应助小绵羊采纳,获得10
18秒前
田様应助小绵羊采纳,获得10
18秒前
18秒前
czp发布了新的文献求助10
19秒前
彩虹马发布了新的文献求助10
19秒前
蓝胖子发布了新的文献求助10
20秒前
年轻水壶完成签到 ,获得积分10
21秒前
23秒前
斯文败类应助地精术士采纳,获得10
23秒前
小美最棒发布了新的文献求助10
23秒前
李铛铛发布了新的文献求助10
25秒前
852应助取法乎上采纳,获得10
27秒前
彩虹马完成签到,获得积分10
28秒前
不爱吃banana的猴子完成签到,获得积分10
29秒前
30秒前
欢呼老鼠给欢呼老鼠的求助进行了留言
30秒前
31秒前
天天快乐应助小钧采纳,获得30
32秒前
32秒前
健忘沛文发布了新的文献求助20
32秒前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3051899
求助须知:如何正确求助?哪些是违规求助? 2709225
关于积分的说明 7416342
捐赠科研通 2353554
什么是DOI,文献DOI怎么找? 1245569
科研通“疑难数据库(出版商)”最低求助积分说明 605799
版权声明 595870