小桶
苦瓜
金黄色葡萄球菌
生物
转录组
生物膜
化学
基因
生物化学
微生物学
植物
基因表达
细菌
遗传学
作者
Hong Peng,Ying-Si Wang,Jie Wang,Sujuan Li,Tairen Sun,Tong Liu,Qingshan Shi,Gang Zhou,Xiaobao Xie
出处
期刊:Polish Journal of Microbiology
[Polish Society of Microbiologists]
日期:2021-10-29
卷期号:70 (4): 447-459
被引量:2
标识
DOI:10.33073/pjm-2021-041
摘要
Staphylococcus aureus is the causative agent of numerous and varied clinical infections. Crude aqueous extracts of Melia azedarach fruits inhibit the planktonic growth and initial biofilm formation of S. aureus in a dose-dependent manner. Moreover, the biofilm topologies became sparse and decreased as the concentration of the aqueous extracts increased. RNA-Seq analyses revealed 532 differentially expressed genes (DEGs) after S. aureus exposure to 0.25 g/ml extracts; 319 of them were upregulated, and 213 were downregulated. The majority of DEGs were categorized into abundant sub-groups in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Finally, untargeted UHPLC-MS/MS analyses of the aqueous extracts of M. azedarach fruits demonstrated a highly complex profile in positive and negative electrospray ionization modes. The extracts primarily consisted of lipids and lipid-like molecules, organic acids and their derivatives, phenylpropanoids, polyketides, organoheterocyclic compounds, and benzenoids annotated by abundant lipid maps and KEGG pathways. Overall, this study provides evidences that the aqueous extracts of M. azedarach fruits can control S. aureus infections and sought to understand the mode of action of these extracts on S. aureus.
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