生物
数量性状位点
遗传学
植物抗病性
单核苷酸多态性
人口
基因座(遗传学)
栽培
基因
等位基因
基因型
园艺
医学
环境卫生
作者
Li Long,Fangjie Yao,Fangnian Guan,Yukun Cheng,Luyao Duan,Xuyang Zhao,Hao Li,Zhien Pu,Wei Li,Qiantao Jiang,Yuming Wei,Jian Ma,Houyang Kang,Shoufen Dai,Pengfei Qi,Qiang Xu,Mei Deng,Yu Zheng,Yunfeng Jiang,Guoyue Chen
出处
期刊:Phytopathology
[Scientific Societies]
日期:2021-09-01
卷期号:111 (9): 1594-1601
被引量:13
标识
DOI:10.1094/phyto-10-20-0465-r
摘要
Chinese wheat landrace Anyuehong (AYH) has displayed high levels of stable adult plant resistance (APR) to stripe rust for >15 years. To identify quantitative trait loci (QTLs) for stripe rust resistance in AYH, a set of 110 recombinant inbred lines (RILs) was developed from a cross between AYH and susceptible cultivar Taichung 29. The parents and RILs were evaluated for final disease severity (FDS) in six field tests with a mixture of predominant Puccinia striiformis f. sp. tritici races at the adult plant stage and genotyped via the wheat 55K single-nucleotide polymorphism (SNP) array to construct a genetic map with 1,143 SNP markers. Three QTLs, designated as QYr.AYH-1AS, QYr.AYH-5BL, and QYr.AYH-7DS, were mapped on chromosome 1AS, 5BL, and 7DS, respectively. RILs combining three QTLs showed significantly lower FDS compared with the lines in other combinations. Of them, QYr.AYH-5BL and QYr.AYH-7DS were stably detected in all environments, explaining 13.6 to 21.4% and 17.6 to 33.6% of phenotypic variation, respectively. Compared with previous studies, QYr.AYH-5BL may be a new QTL, whereas QYr.AYH-7DS may be Yr18. Haplotype analysis revealed that QYr.AYH-5BL is probably present in 6.2% of the 323 surveyed Chinese wheat landraces. The kompetitive allele specific PCR (KASP) markers for QYr.AYH-5BL were developed by the linked SNP markers to successfully confirm the effects of the QTL in a validation population derived from a residual heterozygous line and were further assessed in 38 Chinese wheat landraces and 92 cultivars. Our results indicated that QYr.AYH-5BL with linked KASP markers has potential value for marker-assisted selection to improve stripe rust resistance in breeding programs.
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