Fate of PAHs in treated wastewater reused as irrigation water: Environmental risks in water-soil-ryegrass multimedia system

The main aim of this study was to determine the fate, bio-metabolism and environmental risk of low-ring and high-ring polycyclic aromatic hydrocarbons (PAHs) in a water-soil-ryegrass multi-media system, under long-term irrigation condition with micro-polluted treated wastewater. Field experiments were carried out to simulate garden irrigation using treated wastewater containing typical representative low-ring naphthalene (Nap) and high-ring benzo[a]pyrene (BaP). The results showed that BaP's vertical attenuation rate and adsorption accumulation rate were 1.7 and 1.2 times higher than Nap's, respectively. The adsorption, biodegradation, and the rhizosphere effect were responsible for 40.7%, 28.4%, 21.6%, and 30.5%, 36.6%, 17.7%, respectively, of the attenuation of BaP and Nap. The major metabolic pathways of Nap and BaP are hydroxylation, ring opening cleavage, and decarboxylation, with the metabolic chain of BaP being longer than that of Nap due to more ring cleaving reactions. Pseudomonas, Mycobacterium, and Sphingomonas were the functional microorganisms with PAHs degradation capacity that were positively correlated with PAHs degradation, particularly in the rhizosphere. After ten years of irrigation with treated wastewater, the prediction of environmental risk revealed that there were few potential risks. Thus, the results of this feasibility study demonstrated that using treated wastewater for garden irrigation was a relatively safe and effective strategy.