Phosphate functionalized iron based nanomaterials coupled with phosphate solubilizing bacteria as an efficient remediation system to enhance lead passivation in soil

Bioremediation technology has attracted increasing interest due to it efficient, economical and eco-friendly to apply to heavy metal contaminated soil. This study presents a new biological remediation system with phosphate functionalized iron-based nanomaterials and phosphate solubilizing bacterium strain Leclercia adecarboxylata . Different phosphate content functionalized iron-based nanomaterials were prepared, and nZVI@C/P1 (nP: nFe: nC=1:10:200) with high passivation efficiency was selected to combine with PSB for the remediation experiments. The change in lead fraction and microbial community under five conditions (CK, PSB, nZVI@C, nZVI@C/P1, nZVI@C/P1 + PSB) during 10 days incubation were investigate. The results indicated that nZVI@C/P1 + PSB increased the residual fraction of lead by 93.94% compared with the control group. Meanwhile, inoculation of Leclercia adecarboxylata became the dominant microflora in the soil microbial community during the remediation time, improving the utilization rate of phosphate in nZVI@C/P1 and enhancing the passivation efficiency of lead. Experimental findings demonstrated that combining nZVI@C/P1 with PSB could be considered as an efficient strategy for the lead contaminated soil remediation. • More than 99.87% of lead was passivated by nZVI@C/P1 + PSB in liquid medium. • The maximum residual percentage of lead increased by 93.94% compared with CK. • nZVI@C/P1 reduced the toxicity of Pb 2+ to PSB by reduction and precipitation. • Biomineralization is the dominant mechanism to Pb 2+ passivation in defined remediation system.