Cell-laden Polymeric Microspheres for Biomedical Applications

微球 细胞 生物分子 体外 移植 体内 纳米技术 细胞培养 化学 细胞生物学 生物物理学 材料科学 生物 生物化学 生物技术 化学工程 医学 遗传学 外科 工程类
作者
Wenyan Leong,Dong‐An Wang
出处
期刊:Trends in Biotechnology [Elsevier BV]
卷期号:33 (11): 653-666 被引量:94
标识
DOI:10.1016/j.tibtech.2015.09.003
摘要

Polymers for cell-laden microspheres are discussed. Fabrication techniques and key parameters regarding cell-laden microspheres for specific applications are explored. Several uses of cell-laden microspheres (in vitro production of cells and biomolecules, as well as in vivo delivery of cells for tissue regeneration or therapeutic biomolecule secretion on-site) are discussed and summarized. Microsphere technology serves as an efficient and effective platform for cell applications (in vitro cell culture and in vivo cell delivery) due to its mimicry of the 3D native environment, high surface area:volume ratio, and ability to isolate the entrapped cells from the environment. Properties of cell-laden microspheres are determined by the type of application and the cell. While high cell densities are preferable for large-scale therapeutic biomolecule production in vitro, an immunoprotective barrier is most important for allogeneic pancreatic islet transplantation into patients. Furthermore, the biological cells require a suitable microenvironment in terms of its physical and biochemical properties. Here, we discuss applications of cell-laden microspheres and their corresponding design parameters. Microsphere technology serves as an efficient and effective platform for cell applications (in vitro cell culture and in vivo cell delivery) due to its mimicry of the 3D native environment, high surface area:volume ratio, and ability to isolate the entrapped cells from the environment. Properties of cell-laden microspheres are determined by the type of application and the cell. While high cell densities are preferable for large-scale therapeutic biomolecule production in vitro, an immunoprotective barrier is most important for allogeneic pancreatic islet transplantation into patients. Furthermore, the biological cells require a suitable microenvironment in terms of its physical and biochemical properties. Here, we discuss applications of cell-laden microspheres and their corresponding design parameters. transplantable product that is sourced from a different individual, but of the same species. a homeostatic process in ADCs to undergo programmed cell death (apoptosis) when there is insufficient, inappropriate, or no adhesion to a substrate. This mechanism is the innate switch of a cell to undergo apoptosis upon detachment so as to avert dysplastic proliferation, a key step in tumor formation and metastasis. cells that require extensive adhesion to a substrate and exhibit a spreading morphology; includes most cell types, including endothelial, neuronal, and muscle cells. the process of cells losing their typical morphology and their cell-specific functions when cultured in a monolayer long-term. the process of stem cells changing to a downstream, specialized cell type. end-stage inflammatory response characterized by fusion of macrophages on a biomaterial surface and fibrous capsule formation as part of wound healing. the ability of a compound to induce a humoral (antibody-mediated) or cell-mediated immune response a host response towards the implanted material that leads to immune cell activation and production of cytokines. the temperature below which the polymer changes from a gel into a solution. a microcarrier that contains large pores, allowing cells to penetrate and settle inside it. a microsphere that has a protective capsule over its encapsulated biological cells or drugs. a microsphere that presents cells or drugs on its surface. The microsphere is usually fabricated before seeding of cells. cells that do not require (extensive) adhesion to survive and typically have a rounded morphology. They may be colony forming in soft substrates (e.g., chondrocytes and pluripotent stem cells) or in suspension cultures typically comprising cells of the hematopoietic lineage. an amphiphilic molecule (contains a hydrophilic end and a hydrophobic end) that inserts between two immiscible phases of an emulsion, thereby lowering the interfacial tension and stabilizing the emulsion. transplantable product that is sourced from a different species.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Lee发布了新的文献求助10
1秒前
jiya完成签到,获得积分10
2秒前
飘零的歌手完成签到,获得积分10
2秒前
2秒前
斯文败类应助江璃采纳,获得10
3秒前
3秒前
3秒前
英吉利25发布了新的文献求助30
3秒前
瞿娜发布了新的文献求助10
3秒前
PWG发布了新的文献求助30
3秒前
典雅飞飞完成签到,获得积分10
4秒前
失眠朋友发布了新的文献求助10
5秒前
鲜艳的朋友完成签到,获得积分20
5秒前
happyou完成签到,获得积分10
5秒前
自觉的夏蓉完成签到,获得积分10
6秒前
6秒前
妮儿发布了新的文献求助10
6秒前
aa发布了新的文献求助30
6秒前
谦让的慕凝完成签到 ,获得积分10
6秒前
8秒前
辛未完成签到 ,获得积分10
8秒前
小兔子乖乖完成签到,获得积分10
8秒前
9秒前
梓歆完成签到 ,获得积分10
9秒前
9秒前
9秒前
Singularity应助我会开心采纳,获得10
10秒前
10秒前
10秒前
庸人自扰发布了新的文献求助10
11秒前
Ava应助甜美早晨采纳,获得10
11秒前
tbc完成签到,获得积分10
11秒前
刘二宝完成签到,获得积分20
11秒前
12秒前
赵本山完成签到,获得积分20
12秒前
Akim应助Paustino采纳,获得10
13秒前
奥利安费发布了新的文献求助10
13秒前
汉堡包应助like采纳,获得10
13秒前
13秒前
jiya发布了新的文献求助10
14秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3974426
求助须知:如何正确求助?哪些是违规求助? 3518788
关于积分的说明 11195842
捐赠科研通 3254946
什么是DOI,文献DOI怎么找? 1797649
邀请新用户注册赠送积分活动 877037
科研通“疑难数据库(出版商)”最低求助积分说明 806130