Cell-laden Polymeric Microspheres for Biomedical Applications

微球 细胞 生物分子 体外 移植 体内 纳米技术 细胞培养 化学 细胞生物学 生物物理学 材料科学 生物 生物化学 生物技术 化学工程 医学 遗传学 外科 工程类
作者
Wenyan Leong,Dong‐An Wang
出处
期刊:Trends in Biotechnology [Elsevier BV]
卷期号:33 (11): 653-666 被引量:94
标识
DOI:10.1016/j.tibtech.2015.09.003
摘要

Polymers for cell-laden microspheres are discussed. Fabrication techniques and key parameters regarding cell-laden microspheres for specific applications are explored. Several uses of cell-laden microspheres (in vitro production of cells and biomolecules, as well as in vivo delivery of cells for tissue regeneration or therapeutic biomolecule secretion on-site) are discussed and summarized. Microsphere technology serves as an efficient and effective platform for cell applications (in vitro cell culture and in vivo cell delivery) due to its mimicry of the 3D native environment, high surface area:volume ratio, and ability to isolate the entrapped cells from the environment. Properties of cell-laden microspheres are determined by the type of application and the cell. While high cell densities are preferable for large-scale therapeutic biomolecule production in vitro, an immunoprotective barrier is most important for allogeneic pancreatic islet transplantation into patients. Furthermore, the biological cells require a suitable microenvironment in terms of its physical and biochemical properties. Here, we discuss applications of cell-laden microspheres and their corresponding design parameters. Microsphere technology serves as an efficient and effective platform for cell applications (in vitro cell culture and in vivo cell delivery) due to its mimicry of the 3D native environment, high surface area:volume ratio, and ability to isolate the entrapped cells from the environment. Properties of cell-laden microspheres are determined by the type of application and the cell. While high cell densities are preferable for large-scale therapeutic biomolecule production in vitro, an immunoprotective barrier is most important for allogeneic pancreatic islet transplantation into patients. Furthermore, the biological cells require a suitable microenvironment in terms of its physical and biochemical properties. Here, we discuss applications of cell-laden microspheres and their corresponding design parameters. transplantable product that is sourced from a different individual, but of the same species. a homeostatic process in ADCs to undergo programmed cell death (apoptosis) when there is insufficient, inappropriate, or no adhesion to a substrate. This mechanism is the innate switch of a cell to undergo apoptosis upon detachment so as to avert dysplastic proliferation, a key step in tumor formation and metastasis. cells that require extensive adhesion to a substrate and exhibit a spreading morphology; includes most cell types, including endothelial, neuronal, and muscle cells. the process of cells losing their typical morphology and their cell-specific functions when cultured in a monolayer long-term. the process of stem cells changing to a downstream, specialized cell type. end-stage inflammatory response characterized by fusion of macrophages on a biomaterial surface and fibrous capsule formation as part of wound healing. the ability of a compound to induce a humoral (antibody-mediated) or cell-mediated immune response a host response towards the implanted material that leads to immune cell activation and production of cytokines. the temperature below which the polymer changes from a gel into a solution. a microcarrier that contains large pores, allowing cells to penetrate and settle inside it. a microsphere that has a protective capsule over its encapsulated biological cells or drugs. a microsphere that presents cells or drugs on its surface. The microsphere is usually fabricated before seeding of cells. cells that do not require (extensive) adhesion to survive and typically have a rounded morphology. They may be colony forming in soft substrates (e.g., chondrocytes and pluripotent stem cells) or in suspension cultures typically comprising cells of the hematopoietic lineage. an amphiphilic molecule (contains a hydrophilic end and a hydrophobic end) that inserts between two immiscible phases of an emulsion, thereby lowering the interfacial tension and stabilizing the emulsion. transplantable product that is sourced from a different species.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI5应助cc采纳,获得10
1秒前
铜泰妍完成签到 ,获得积分10
2秒前
贝贝完成签到 ,获得积分10
7秒前
Lrcx完成签到 ,获得积分10
8秒前
Wen完成签到 ,获得积分10
9秒前
盘尼西林完成签到 ,获得积分10
11秒前
LOVE0077完成签到,获得积分10
14秒前
zhao完成签到,获得积分10
16秒前
BINBIN完成签到 ,获得积分10
26秒前
ambrose37完成签到 ,获得积分10
28秒前
量子星尘发布了新的文献求助10
34秒前
fufufu123完成签到 ,获得积分10
38秒前
开心的大娘完成签到,获得积分10
38秒前
www完成签到 ,获得积分10
40秒前
末末完成签到 ,获得积分10
50秒前
无为完成签到 ,获得积分10
51秒前
白嫖论文完成签到 ,获得积分10
53秒前
上官若男应助忧伤的步美采纳,获得10
56秒前
科研通AI2S应助科研通管家采纳,获得10
56秒前
59秒前
从心随缘完成签到 ,获得积分10
1分钟前
花花发布了新的文献求助10
1分钟前
牛奶面包完成签到 ,获得积分10
1分钟前
1分钟前
岁月如歌完成签到 ,获得积分0
1分钟前
1分钟前
Li完成签到,获得积分10
1分钟前
张琨完成签到 ,获得积分10
1分钟前
1分钟前
sunnyqqz完成签到,获得积分10
1分钟前
热情的乘风完成签到,获得积分20
1分钟前
1分钟前
霍凡白完成签到,获得积分10
1分钟前
1分钟前
Feng发布了新的文献求助20
1分钟前
怕孤单的若颜完成签到 ,获得积分10
1分钟前
1分钟前
ruochenzu发布了新的文献求助10
1分钟前
zhongu发布了新的文献求助10
1分钟前
阳光彩虹小白马完成签到 ,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038039
求助须知:如何正确求助?哪些是违规求助? 3575756
关于积分的说明 11373782
捐赠科研通 3305574
什么是DOI,文献DOI怎么找? 1819239
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022