Optimization of photobioreactor growth conditions for a cyanobacterium expressing mosquitocidal Bacillus thuringiensis Cry proteins

光合反应器 苏云金杆菌 生物反应器 生物 气流 蓝藻 鱼腥藻 混合营养体 生产力 植物 生物技术 异养 细菌 遗传学 机械工程 宏观经济学 工程类 经济 生物燃料
作者
Irene Ketseoglou,Gustav Bouwer
出处
期刊:Journal of Biotechnology [Elsevier BV]
卷期号:167 (1): 64-71 被引量:7
标识
DOI:10.1016/j.jbiotec.2013.05.012
摘要

An Anabaena strain (PCC 7120#11) that was genetically engineered to express Bacillus thuringiensis subsp. israelensis cry genes has shown good larvicidal activity against Anopheles arabiensis, a major vector of malaria in Africa. Response surface methodology was used to evaluate the relationship between key growth factors and the volumetric productivity of PCC 7120#11 in an indoor, flat-plate photobioreactor. The interaction of input CO₂ concentration and airflow rate had a statistically significant effect on the volumetric productivity of PCC 7120#11, as did the interaction of airflow rate and photosynthetic photon flux density. Model-based numerical optimization indicated that the optimal factor level combination for maximizing PCC 7120#11 volumetric productivity was a photosynthetic photon flux density of 154 μmol m⁻² s⁻¹ and air enriched with 3.18% (v/v) CO₂ supplied at a flow rate of 1.02 vessel volumes per minute. At the levels evaluated in the study, none of the growth factors had a significant effect on the median lethal concentration of PCC 7120#11 against An. arabiensis larvae. This finding is important because loss of mosquitocidal activity under growth conditions that maximize volumetric productivity would impact on the feasibility of using PCC 7120#11 in malaria vector control programs. The study showed the usefulness of response surface methodology for determination of the optimal growth conditions for a cyanobacterium that is genetically engineered to have larvicidal activity against malaria vectors.
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