分析物
比色法
适体
链霉素
RGB颜色模型
检出限
荧光
荧光染料
人工智能
数码相机
化学
色谱法
计算机科学
光学
抗生素
生物
实时聚合酶链反应
分子生物学
生物化学
物理
基因
作者
Bixia Lin,Ying Yu,Ya Cao,Minzhe Guo,Dongmei Zhu,Jiaxing Dai,Minshi Zheng
标识
DOI:10.1016/j.bios.2017.09.028
摘要
The rapid detection of antibiotic residual in everyday life is very important for food safety. In order to realize the on-site and visual detection of antibiotic, a POCT method was established by using digital image colorimetry based on smartphone. Streptomycin was taken as the analyte model of antibiotics, streptomycin aptamer preferentially recognized analyte, and the excess aptamer hybridized with the complementary DNA to form the dsDNA. SYBR Green I combined with the dsDNA and then emitted obvious green fluorescence, thus the fluorescence intensity decreased with the increasing of streptomycin concentration. Then a smartphone-based device was constructed as the fluorescence readout. The smartphone camera acquired the images of the fluorescence derived from the samples, and the Touch Color APP installed in smartphone read out the RGB values of the images. There was a linear relationship between the G values and the streptomycin concentrations in the range of 0.1-100µM. The detection limit was 94nM, which was lower than the maximum residue limit defined by World Health Organization. The POCT method was applied for determining streptomycin in chicken and milk samples with recoveries in 94.1-110%. This method had the advantages of good selectivity, simple operation and on-site visualization.
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