奶油
多巴胺
脑源性神经营养因子
神经营养因子
星形胶质细胞
生物
内科学
神经科学
多巴胺受体
内分泌学
下调和上调
受体
细胞生物学
转录因子
医学
中枢神经系统
生物化学
基因
作者
Indrek Koppel,Kaur Jaanson,Airi Klasche,Jürgen Tuvikene,Tõnis Tiirik,Angela Pärn,Tõnis Timmusk
出处
期刊:Glia
[Wiley]
日期:2017-10-06
卷期号:66 (1): 206-216
被引量:44
摘要
Expression of brain-derived neurotrophic factor (BDNF) is induced in cultured rat cortical astrocytes by catecholamines norepinephrine and dopamine as well as selective α1 and β adrenergic agonists. However, it has remained unknown which receptors mediate dopamine-induced BDNF upregulation in astrocytes. Here, we demonstrate that β adrenoreceptors are the main mediators of this effect in cultured cortical astrocytes, while α1 adrenoreceptors and D1 dopamine receptors contribute to a lesser extent. We show that in cortical astrocytes BDNF exon IV and exon VI containing mRNAs are induced by dopamine and norepinephrine via CREB-dependent signaling and that this regulation is mediated by a mechanism that is distinct from activity-dependent CREB-mediated activation of BDNF transcription in neurons. We also show that regulation of BDNF promoters IV and VI by catecholamines requires a distal regulatory element in the BDNF locus. Finally, we demonstrate that dopamine-induced astrocyte stellation and induction of CREB signaling are mediated by cross-reaction of dopamine with β adrenoreceptors.
科研通智能强力驱动
Strongly Powered by AbleSci AI