代谢组学
化学
质谱法
鉴定(生物学)
分析物
计算生物学
样品制备
代谢物分析
样品(材料)
色谱法
生物
植物
作者
Katja Dettmer,Pavel A. Aronov,Bruce D. Hammock
摘要
Abstract This review presents an overview of the dynamically developing field of mass spectrometry‐based metabolomics. Metabolomics aims at the comprehensive and quantitative analysis of wide arrays of metabolites in biological samples. These numerous analytes have very diverse physico‐chemical properties and occur at different abundance levels. Consequently, comprehensive metabolomics investigations are primarily a challenge for analytical chemistry and specifically mass spectrometry has vast potential as a tool for this type of investigation. Metabolomics require special approaches for sample preparation, separation, and mass spectrometric analysis. Current examples of those approaches are described in this review. It primarily focuses on metabolic fingerprinting, a technique that analyzes all detectable analytes in a given sample with subsequent classification of samples and identification of differentially expressed metabolites, which define the sample classes. To perform this complex task, data analysis tools, metabolite libraries, and databases are required. Therefore, recent advances in metabolomics bioinformatics are also discussed. © 2006 Wiley Periodicals, Inc., Mass Spec Rev 26:51–78, 2007
科研通智能强力驱动
Strongly Powered by AbleSci AI