Expression Vector Construction of Strawberry Ers1 Gene

[Objective] To construct the antisense expression vector of strawberry Ers1 gene,in order to explore the function of the gene and use the biological technology to improve the strawberry cultivars.[Method] According to the restriction enzyme sites of expression vector and cloning sequence of strawberry Ers1 gene,one pair of primers containing restriction enzyme sites were designed and used to amplify sequenced plasmid.PCR product and the plasmid pBI121 were digested by the corresponding restricted enzymes respectively,and linked directionally.Then the antisense expression vector can be designed.[Result] Through restriction enzyme identification,the antisense plant expression vector of strawberry Ers1 gene was successfully constructed.[Conclusion] The study laid foundation for further discussing the functions of Ers1 gene,regulating the fruits maturing process via biotechnological method and improving the storage and preservation performance of strawberries.