[Isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans].

To study the methods of extraction, isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans (AEG).AEG was purified by distilled water extraction, ethanol fractionation, Sephadex column chromatography. The purity and molecular weight and concentration of AEG were measured by HPLC; We divided the experiment into Physiological Saline group and the other eight groups with different doses of AEG, established RT-PRC method to observe the anti-HPV effect after their actions on the verruca tissues.Using HPLC, the group of AEG was divided into two glycosaminoglycans with different molecular weight as 27336 and 1152. Bioassay results showed that AEG had anti-HPV-DNA activity, the lowest effective concentration was 0.781 mg/ml.AEG extracted by this method is a mixture with two molecular weight glycosaminoglycans, and has obvious anti-HPV effect.