Secondary metabolites of marine fungus Eutypella scoparia from the South China Sea and their antitumor activities

Objective To study the secondary metabolites of the marine fungus Eutypella scoparia from the South China Sea and their antitumor activities.Methods The compounds were isolated by various chromatographic methods(silica gel,reverse silica gel,Sephadex-LH20,preparative TLC,and so on) and recrystallization.Their structures were identified by extensive analysis of their spectroscopic data.The inhibitory effects of these compounds on SF-268,MCF-7,and NCI-H460 cell lines were observed in vitro by MTT method.Results Ten compounds(six pimarane diterpenes and four steroids) were isolated from the culture and identified as isopimara-8(14),15-diene(1),libertellenone A(2),scopararane B(3),diaporthein A(4),diaporthein B(5),11-deoxydiaporthein A(6),(22E,24R)-ergosta-4,6,8(14)-22-tetraen-3-one(7),ergosterol(8),ergosterol peroxide(9),and cerevisterol(10).Compound 5 has the notable cytotoxic activities on SF-268,MCF-7,and NCI-H460 cell growth with IC50 values of 9.2,4.4,and 9.9 μmol/L,respectively.Conclusion Compounds 1,2,and 6—10 are isolated from the fungi of Eutypella(Nits.) Sacc.for the first time and compound 5 demonstrates the significant inhibition on above three cell lines.