细胞凋亡
白色念珠菌
标记法
生物
程序性细胞死亡
蛋白质组学
白色体
细胞生物学
半胱氨酸蛋白酶
细胞
生物化学
分子生物学
微生物学
基因
作者
Virginia Cabezón,Vital Vialás,Ana Gil-Bona,José Antonio Reales-Calderón,Montserrat Martínez‐Gomariz,Dolores Gutiérrez-Blázquez,Lucía Monteoliva,Gloria Molero,Mark Ramsdale,Concha Gil
标识
DOI:10.1021/acs.jproteome.5b00913
摘要
Macrophages may induce fungal apoptosis to fight against C. albicans, as previously hypothesized by our group. To confirm this hypothesis, we analyzed proteins from C. albicans cells after 3 h of interaction with macrophages using two quantitative proteomic approaches. A total of 51 and 97 proteins were identified as differentially expressed by DIGE and iTRAQ, respectively. The proteins identified and quantified were different, with only seven in common, but classified in the same functional categories. The analyses of their functions indicated that an increase in the metabolism of amino acids and purine nucleotides were taking place, while the glycolysis and translation levels dropped after 3 h of interaction. Also, the response to oxidative stress and protein translation were reduced. In addition, seven substrates of metacaspase (Mca1) were identified (Cdc48, Fba1, Gpm1, Pmm1, Rct1, Ssb1, and Tal1) as decreased in abundance, plus 12 proteins previously described as related to apoptosis. Besides, the monitoring of apoptotic markers along 24 h of interaction (caspase-like activity, TUNEL assay, and the measurement of ROS and cell examination by transmission electron microscopy) revealed that apoptotic processes took place for 30% of the fungal cells, thus supporting the proteomic results and the hypothesis of macrophages killing C. albicans by apoptosis.
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