Apoptosis of Candida albicans during the Interaction with Murine Macrophages: Proteomics and Cell-Death Marker Monitoring

细胞凋亡 白色念珠菌 标记法 生物 程序性细胞死亡 蛋白质组学 白色体 细胞生物学 半胱氨酸蛋白酶 细胞 生物化学 分子生物学 微生物学 基因
作者
Virginia Cabezón,Vital Vialás,Ana Gil-Bona,José Antonio Reales-Calderón,Montserrat Martínez‐Gomariz,Dolores Gutiérrez-Blázquez,Lucía Monteoliva,Gloria Molero,Mark Ramsdale,Concha Gil
出处
期刊:Journal of Proteome Research [American Chemical Society]
卷期号:15 (5): 1418-1434 被引量:18
标识
DOI:10.1021/acs.jproteome.5b00913
摘要

Macrophages may induce fungal apoptosis to fight against C. albicans, as previously hypothesized by our group. To confirm this hypothesis, we analyzed proteins from C. albicans cells after 3 h of interaction with macrophages using two quantitative proteomic approaches. A total of 51 and 97 proteins were identified as differentially expressed by DIGE and iTRAQ, respectively. The proteins identified and quantified were different, with only seven in common, but classified in the same functional categories. The analyses of their functions indicated that an increase in the metabolism of amino acids and purine nucleotides were taking place, while the glycolysis and translation levels dropped after 3 h of interaction. Also, the response to oxidative stress and protein translation were reduced. In addition, seven substrates of metacaspase (Mca1) were identified (Cdc48, Fba1, Gpm1, Pmm1, Rct1, Ssb1, and Tal1) as decreased in abundance, plus 12 proteins previously described as related to apoptosis. Besides, the monitoring of apoptotic markers along 24 h of interaction (caspase-like activity, TUNEL assay, and the measurement of ROS and cell examination by transmission electron microscopy) revealed that apoptotic processes took place for 30% of the fungal cells, thus supporting the proteomic results and the hypothesis of macrophages killing C. albicans by apoptosis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
李爱国应助hucong采纳,获得10
1秒前
从容芮应助Kelly采纳,获得30
1秒前
彳亍1117应助小胡爱学习采纳,获得20
2秒前
stuffmatter应助冷傲山灵采纳,获得50
2秒前
2秒前
3秒前
王也笑然发布了新的文献求助10
4秒前
华仔应助活力的亦云采纳,获得10
5秒前
英俊的铭应助小北采纳,获得10
6秒前
JamesPei应助十五采纳,获得10
6秒前
6秒前
可爱的函函应助LXZ采纳,获得10
6秒前
7秒前
上官若男应助CY采纳,获得10
8秒前
8秒前
汪鸡毛完成签到 ,获得积分10
8秒前
李爱国应助半夏采纳,获得10
8秒前
uziMOF发布了新的文献求助10
8秒前
薰硝壤应助30采纳,获得50
8秒前
9秒前
zzzz发布了新的文献求助10
10秒前
wen应助星星采纳,获得20
11秒前
葫芦发布了新的文献求助50
11秒前
小闹发布了新的文献求助10
11秒前
12秒前
13秒前
15秒前
15秒前
东丶完成签到,获得积分10
15秒前
15秒前
彭于晏应助逢考必过采纳,获得10
17秒前
俏皮的一德完成签到,获得积分10
18秒前
18秒前
CY发布了新的文献求助10
18秒前
19秒前
大大小小发布了新的文献求助10
20秒前
夹心饼干关注了科研通微信公众号
20秒前
21秒前
CoCoco完成签到,获得积分10
21秒前
拼搏老九发布了新的文献求助10
22秒前
高分求助中
Impact of Mitophagy-Related Genes on the Diagnosis and Development of Esophageal Squamous Cell Carcinoma via Single-Cell RNA-seq Analysis and Machine Learning Algorithms 2000
Evolution 1500
How to Create Beauty: De Lairesse on the Theory and Practice of Making Art 1000
Gerard de Lairesse : an artist between stage and studio 670
CLSI EP47 Evaluation of Reagent Carryover Effects on Test Results, 1st Edition 550
Decision Theory 500
Multiscale Thermo-Hydro-Mechanics of Frozen Soil: Numerical Frameworks and Constitutive Models 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 2988343
求助须知:如何正确求助?哪些是违规求助? 2649526
关于积分的说明 7158953
捐赠科研通 2283573
什么是DOI,文献DOI怎么找? 1210766
版权声明 592454
科研通“疑难数据库(出版商)”最低求助积分说明 591239