单克隆抗体
溶解
表位
化学
补体成分5
抗体
补体系统
补语(音乐)
补体膜攻击复合物
分子生物学
免疫学
生物
生物化学
基因
表型
互补
作者
Reinhard Würzner,Matthias B. Schulze,Ludger Happe,Anke Franzke,F. Bieber,Martin Oppermann,Otto Götze
出处
期刊:Complement and inflammation
[S. Karger AG]
日期:1991-01-01
卷期号:8 (5-6): 328-340
被引量:84
摘要
Three monoclonal antibodies (mabs), two against C5 and one against C6, were identified and characterized. They inhibited the generation of the terminal complement complex (TCC) in serum to over 90% as assayed by a sensitive ELISA based on a neoepitope- specific mab, which recognized TCC-integrated C9. The haemolytic function of the TCC was markedly reduced by all three mabs implying that they are directed to epitopes on C5 and C6 which are essential for TCC formation in both the fluid phase and on erythrocyte membranes. Since the generation of C5a was also impaired by these mabs, they may serve as tools in investigations of the sequelae of the generation of C5a and of TCC.
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