RNA聚合酶Ⅰ
核糖核酸
分子生物学
RNA聚合酶
抄写(语言学)
基因
RNA依赖性RNA聚合酶
小RNA
RNA聚合酶Ⅲ
聚合酶
非编码RNA
作者
Yoontae Lee,Minju Kim,Jinju Han,Kyu Hyun Yeom,Sanghyuk Lee,Sung Hee Baek,V. Narry Kim
标识
DOI:10.1038/sj.emboj.7600385
摘要
MicroRNAs (miRNAs) constitute a large family of noncoding RNAs that function as guide molecules in diverse gene silencing pathways. Current efforts are focused on the regulatory function of miRNAs, while little is known about how these unusual genes themselves are regulated. Here we present the first direct evidence that miRNA genes are transcribed by RNA polymerase II (pol II). The primary miRNA transcripts (pri‐miRNAs) contain cap structures as well as poly(A) tails, which are the unique properties of class II gene transcripts. The treatment of human cells with α‐amanitin decreased the level of pri‐miRNAs at a concentration that selectively inhibits pol II activity. Furthermore, chromatin immunoprecipitation analyses show that pol II is physically associated with a miRNA promoter. We also describe, for the first time, the detailed structure of a miRNA gene by determining the promoter and the terminator of mir‐23a∼27a∼24‐2 . These data indicate that pol II is the main, if not the only, RNA polymerase for miRNA gene transcription. Our study offers a basis for understanding the structure and regulation of miRNA genes.
科研通智能强力驱动
Strongly Powered by AbleSci AI