生物
脂滴
细胞生物学
生物发生
细胞器
细胞器生物发生
伴侣(临床)
酵母
脂质代谢
未折叠蛋白反应
磷脂
酿酒酵母
生物化学
内质网
膜
基因
病理
医学
作者
Jason Vevea,Enrique J. García,Robin Chan,Bowen Zhou,Mei Schultz,Gilbert Di Paolo,J. Michael McCaffery,Enrique J. García
标识
DOI:10.1016/j.devcel.2015.11.010
摘要
The immediate responses to inhibition of phosphatidylcholine (PC) biosynthesis in yeast are altered phospholipid levels, slow growth, and defects in the morphology and localization of ER and mitochondria. With chronic lipid imbalance, yeast adapt. Lipid droplet (LD) biogenesis and conversion of phospholipids to triacylglycerol are required for restoring some phospholipids to near-wild-type levels. We confirmed that the unfolded protein response is activated by this lipid stress and find that Hsp104p is recruited to ER aggregates. We also find that LDs form at ER aggregates, contain polyubiquitinated proteins and an ER chaperone, and are degraded in the vacuole by a process resembling microautophagy. This process, microlipophagy, is required for restoration of organelle morphology and cell growth during adaptation to lipid stress. Microlipophagy does not require ATG7 but does requires ESCRT components and a newly identified class E VPS protein that localizes to ER and is upregulated by lipid imbalance.
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