生物
EC50型
细胞外
拉伤
生物测定
活性氧
体外
生物化学
微生物学
毒理
解剖
生态学
作者
Guangxun Du,Chunxiao Sun,Yankai Liu,Qing Dong,Ping Gao,Dehai Li,Lingyun Qu
出处
期刊:Aquaculture
[Elsevier]
日期:2021-03-01
卷期号:534: 736337-736337
被引量:2
标识
DOI:10.1016/j.aquaculture.2020.736337
摘要
Uronema marinum, a well-known pathogenic parasite found in various marine fish species, caused severe economic losses in the field of aquaculture. This study aimed to assess the anti-parasitic activity and clarify the anti-parasitic mechanism of active compounds isolated from the extracellular product of Salinivibrio proteolyticus strain YCSC6 against U. marinum in vitro. Two β-carboline derivatives (βCs), harmane and norharmane, were isolated from the extracellular product of strain YCSC6 through the bioassay-guided fractionation. According to the results, harmane could eliminate U. marinum at 6 mg L−1 after 48 h exposure or 12 mg L−1 after 4 h exposure. Conversely, norharmane could eliminate U. marinum at 14 mg L−1 after 48 h exposure or 38 mg L−1 after 4 h exposure. The median effective concentration (EC50) of harmane after 4, 8, 12, 24 and 48 h exposure was 5.51±0.21, 3.63±0.08, 3.53±0.25, 2.87±0.12 and 2.47±0.12 mg L−1, and that of norharmane was 24.44±0.10, 16.63±0.14, 13.24±0.11, 6.50±0.02 and 4.53±0.15 mg L−1, respectively. In the aspect of anti-parasitic mechanism, harmane and norharmane transferred the electrons of 2-N to ground-state oxygen forming excessive reactive oxygen species (ROS), which damaged the plasma membrane and DNA of U. marinum. Meanwhile, the structure-activity relationship (SAR) analysis indicated 2-N was the necessary site for βCs to exert anti-parasitic activity and the 1-methyl substitution could enhance the anti-parasitic activity. Our findings indicated that harmane and norharmane could be considered as promising lead compounds for developing novel and highly-effective anti-parasitic agents for aquaculture.
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