注意事项
材料科学
病毒
纳米技术
微流控
纳米棒
检出限
检测点注意事项
病毒学
生物
化学
医学
色谱法
护理部
免疫学
作者
Yiqiu Xia,Yizhu Chen,Yeming Tang,Gong Cheng,Xu Yu,Hong‐Zhang He,Guohong Cao,Huaguang Lu,Zhiwen Liu,Siyang Zheng
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2019-11-26
卷期号:4 (12): 3298-3307
被引量:77
标识
DOI:10.1021/acssensors.9b01927
摘要
Viruses pose serious infectious disease threats to humans and animals. To significantly decrease the mortality and morbidity caused by virus infections, there is an urgent need of sensitive and rapid point-of-care platforms for virus detection, especially in low-resource settings. Herein, we developed a smartphone-based point-of-care platform for highly sensitive and selective detection of the avian influenza virus based on nanomaterial-enabled colorimetric detection. The 3D nanostructures, which serve as a scaffold for antibody conjugation to capture the avian influenza virus, are made on PDMS herringbone structures with a ZnO nanorod template. After virus capture, the on-chip gold nanoparticle-based colorimetric reaction allows virus detection by naked eyes with a detection limit of 2.7 × 104 EID50/mL, which is one order of magnitude better than that of conventional fluorescence-based ELISA. Furthermore, a smartphone imaging system with data processing capability further improves the detection limit, reaching down to 8 × 103 EID50/mL. The entire virus capture and detection process can be completed in 1.5 h. We envision that this point-of-care microfluidic system integrated with smartphone imaging and colorimetric detection would provide a fast, cheap, sensitive, and user-friendly platform for virus detection in low-resource settings.
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