Comparison of the effect on proliferation of osteoblasts MC3T3-E1 between the concentrated growth factor extract and the platelet-rich fibrin extract.

To compare the effect on proliferation of osteoblasts MC3T3-E1 between the concentrated growth factor extract (CGFe) and the platelet-rich fibrin extract (PRFe).CGFe and PRFe were prepared. MC3T3-E1 was cultured in DMEM medium containing CGFe (10%, 20%, or 30%) and PRFe (10%, 20%, or 30%). The proliferation of MC3T3-E1 was detected by MTT assay at Day 1, 3, 5, and 7. ALP activity was detected by alkaline phosphatase (ALP) staining at Day 1, 3, 5, and 7, and mRNA expressions of Runt-related transcription factor 2 (Runx2) and Osterix (Osx) were detected by quantitative RT-PCR (RT-qPCR) at Day 3 and 7.Compared with the control group, CGFe and PRFe promoted the proliferation of MC3T3-E1 at Day 1, 3, 5, and 7 (all P<0.05). Except for the first day, the proliferation activity in the CGFe group was higher than that in the PRFe group (all P<0.05). At Day 1, 3, 5, and 7, compared with the control group, the ALP activities in the CGFe group and the PRFe group were significantly increased (all P<0.05). Except for the first day, the ALP activity in the CGFe group was higher than that in the PRFe group (all P<0.05). At Day 3 and 7, compared with the control group, the mRNA expression levels of Osx and Runx2 in the CGFe group and the PRFe group were significantly increased (all P<0.05); compared with PRFe group, the mRNA expression level of Osx in the CGFe group was significantly higher than that in the PRFe group, and the mRNA expression level of Runx2 was significantly lower than that in the PRFe group (all P<0.05).CGFe could promote the proliferation of MC3T3-E1 stronger than PRFe, which might be related to the increase of ALP activity and up-regulation of Osx expression.目的: 比较浓缩生长因子提取液(concentrated growth factor extract，CGFe)和富血小板纤维蛋白提取液(platelet-rich fibrin extract，PRFe)对成骨细胞MC3T3-E1增殖的影响。方法: 制备CGFe和PRFe。分别采用含CGFe(10%，20%，30%)与PRFe(10%，20%，30%)的DMEM培养基培养MC3T3-E1。分别于培养的第1，3，5，7天，采用MTT法检测细胞增殖情况，碱性磷酸酶(alkaline phosphatase，ALP)染色法检测细胞中ALP的活性；采用定量RT-PCR(quantitative RT-PCR，RT-qPCR)检测培养第3和7天细胞中核心结合蛋白因子2(Runt-related transcription factor 2，Runx2)和成骨细胞特异性转录因子(Osterix，Osx)mRNA表达水平。结果: 在1，3，5，7 d时，与对照组相比，CGFe与PRFe均能促进成骨细胞MC3T3-E1的增殖，差异有统计学意义(均P<0.05)。除第1天外，相同浓度的CGFe与PRFe比较，CGFe组细胞的增殖活性高于PRFe组，差异有统计学差异(均P<0.05)。在1，3，5，7 d时，与对照组相比，CGFe组与PRFe组的ALP活性均明显升高，差异有统计学意义(均P<0.05)。除第1天外，相同浓度的CGFe组与PRFe组比较，CGFe组的ALP活性高于PRFe组，差异有统计学意义(均P<0.05)。在3，7 d时，与对照组相比，CGFe组与PRFe组Osx和Runx2 mRNA表达水平均明显升高，差异有统计学意义(均P<0.05)；相同浓度的CGFe组与PRFe组比较，CGFe组Osx mRNA表达水平明显高于PRFe组，Runx2 mRNA表达水平明显低于PRFe组，差异有统计学意义(均P<0.05)。结论: CGFe比PRFe更能促进成骨细胞MC3T3-E1的增殖，这可能与其增加ALP活性及上调成骨相关基因Osx的表达有关。.