Unripe Rubus coreanus Miquel Extract Containing Ellagic Acid Regulates AMPK, SREBP-2, HMGCR, and INSIG-1 Signaling and Cholesterol Metabolism In Vitro and In Vivo

鞣花酸 安普克 胆固醇 化学 生物化学 药理学 磷酸化 蛋白激酶A 生物 抗氧化剂 多酚
作者
Ki Hoon Lee,Eui-seon Jeong,Goeun Jang,Ju-Ryun Na,Soyi Park,Wan Seok Kang,Eun Sun Kim,Hakjoon Choi,Jin Seok Kim,Sunoh Kim
出处
期刊:Nutrients [MDPI AG]
卷期号:12 (3): 610-610 被引量:27
标识
DOI:10.3390/nu12030610
摘要

Our previous study demonstrated that a 5% ethanol extract of unripe Rubus coreanus (5-uRCK) has hypo-cholesterolemic and anti-obesity activity. However, the molecular mechanisms of its effects are poorly characterized. We hypothesized that 5-uRCK and one of its major bioactive compounds, ellagic acid, decrease cellular and plasma cholesterol levels. Thus, we investigated the hypocholesterolemic activity and mechanism of 5-uRCK in both hepatocytes and a high-cholesterol diet (HCD)-induced rat model. Cholesterol in the liver and serum was significantly reduced by 5-uRCK and ellagic acid. The hepatic activities of HMG-CoA and CETP were reduced, and the hepatic activity of LCAT was increased by both 5-uRCK extract and ellagic acid, which also caused histological improvements. The MDA content in the aorta and serum was significantly decreased after oral administration of 5-uRCK or ellagic acid. Further immunoblotting analysis showed that AMPK phosphorylation in the liver was induced by 5-uRCK and ellagic acid, which activated AMPK, inhibiting the activity of HMGCR by inhibitory phosphorylation. In contrast, 5-uRCK and ellagic acid suppressed the nuclear translocation and activation of SREBP-2, which is a key transcription factor in cholesterol biosynthesis. In conclusion, our results suggest that 5-uRCK and its bioactive compound, ellagic acid, are useful alternative therapeutic agents to regulate blood cholesterol.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
天空之城发布了新的文献求助10
刚刚
包笑白发布了新的文献求助10
刚刚
完美世界应助似水流年采纳,获得10
刚刚
刚刚
月月发布了新的文献求助50
1秒前
jack1完成签到,获得积分10
1秒前
2秒前
ding应助OIIII采纳,获得10
2秒前
赘婿应助wu采纳,获得10
2秒前
气945发布了新的文献求助10
2秒前
2秒前
2秒前
2秒前
xsc发布了新的文献求助10
3秒前
科研通AI2S应助niuniu采纳,获得10
3秒前
博士小学生完成签到,获得积分10
3秒前
啊啊啊完成签到,获得积分10
3秒前
六点一横完成签到,获得积分10
4秒前
PositiveJugend完成签到,获得积分10
4秒前
4秒前
烂漫的雅容完成签到,获得积分10
5秒前
zyx999完成签到,获得积分20
5秒前
5秒前
5秒前
彪yu完成签到 ,获得积分10
6秒前
6秒前
cannon8发布了新的文献求助30
6秒前
宓凌青完成签到,获得积分10
6秒前
7秒前
Docgyj完成签到 ,获得积分10
7秒前
研友_VZG7GZ应助纳斯达克采纳,获得10
7秒前
小李叭叭完成签到,获得积分10
8秒前
威武的翠安完成签到 ,获得积分10
8秒前
zyx999发布了新的文献求助10
8秒前
8秒前
老实的半山完成签到,获得积分10
9秒前
dwhnx完成签到,获得积分10
9秒前
winfree完成签到 ,获得积分10
9秒前
9秒前
月月发布了新的文献求助50
9秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3245464
求助须知:如何正确求助?哪些是违规求助? 2889085
关于积分的说明 8256869
捐赠科研通 2557437
什么是DOI,文献DOI怎么找? 1386114
科研通“疑难数据库(出版商)”最低求助积分说明 650285
邀请新用户注册赠送积分活动 626541