Two loss‐of‐function alleles of the glutathione S‐transferase (GST) gene cause anthocyanin deficiency in flower and fruit skin of peach (Prunus persica)

生物 花青素 遗传学 李子 等位基因 基因 谷胱甘肽 功能(生物学) 园艺 植物 生物化学
作者
Zhenhua Lu,Hong‐Lin Cao,Lei Pan,Liang Niu,Bin Wei,Guochao Cui,Luwei Wang,Jia‐Long Yao,Wenfang Zeng,Zhiqiang Wang
出处
期刊:Plant Journal [Wiley]
卷期号:107 (5): 1320-1331 被引量:27
标识
DOI:10.1111/tpj.15312
摘要

Flower and fruit colors are important agronomic traits. To date, there is no forward genetic evidence that the glutathione S-transferase (GST) gene is responsible for the white flower color in peach (Prunus persica). In this study, genetic analysis indicated that the white-flower trait is monogenetic, is recessive to the non-white allele, and shows pleiotropic effects with non-white-flowered types. The genetic locus underpinning this trait was mapped onto chromosome 3 between 0.421951 and 3.227115 Mb by using bulked segregant analysis in conjunction with whole-genome sequencing, and was further mapped between 0 and 1.178149 Mb by using the backcross 1 (BC1 ) population. Finally, the locus was fine-mapped within 535.974- and 552.027-kb intervals by using 151 F2 individuals and 75 individuals from a BC1 self-pollinated (BC1 S1 ) population, respectively. Pp3G013600, encoding a GST that is known to transport anthocyanin, was identified within the mapping interval. The analysis of genome sequence data showed Pp3G013600 in white flowers has a 2-bp insertion or a 5-bp deletion in the third exon. These variants likely render the GST non-functional because of early stop codons that reduce the protein length from 215 amino acids to 167 and 175 amino acids, respectively. Genetic markers based on these variants validated a complete correlation between the GST loss-of-function alleles and white flower in 128 peach accessions. This correlation was further confirmed by silencing of Pp3G013600 using virus-induced gene silencing technology, which reduced anthocyanin accumulation in peach fruit. The new knowledge from this study is useful for designing peach breeding programs to generate cultivars with white flower and fruit skin.
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