Two loss‐of‐function alleles of the glutathione S‐transferase (GST) gene cause anthocyanin deficiency in flower and fruit skin of peach (Prunus persica)

生物 花青素 遗传学 李子 等位基因 基因 谷胱甘肽 功能(生物学) 园艺 植物 生物化学
作者
Zhenhua Lu,Hong‐Lin Cao,Lei Pan,Liang Niu,Bin Wei,Guochao Cui,Luwei Wang,Jia‐Long Yao,Wenfang Zeng,Zhiqiang Wang
出处
期刊:Plant Journal [Wiley]
卷期号:107 (5): 1320-1331 被引量:27
标识
DOI:10.1111/tpj.15312
摘要

Flower and fruit colors are important agronomic traits. To date, there is no forward genetic evidence that the glutathione S-transferase (GST) gene is responsible for the white flower color in peach (Prunus persica). In this study, genetic analysis indicated that the white-flower trait is monogenetic, is recessive to the non-white allele, and shows pleiotropic effects with non-white-flowered types. The genetic locus underpinning this trait was mapped onto chromosome 3 between 0.421951 and 3.227115 Mb by using bulked segregant analysis in conjunction with whole-genome sequencing, and was further mapped between 0 and 1.178149 Mb by using the backcross 1 (BC1 ) population. Finally, the locus was fine-mapped within 535.974- and 552.027-kb intervals by using 151 F2 individuals and 75 individuals from a BC1 self-pollinated (BC1 S1 ) population, respectively. Pp3G013600, encoding a GST that is known to transport anthocyanin, was identified within the mapping interval. The analysis of genome sequence data showed Pp3G013600 in white flowers has a 2-bp insertion or a 5-bp deletion in the third exon. These variants likely render the GST non-functional because of early stop codons that reduce the protein length from 215 amino acids to 167 and 175 amino acids, respectively. Genetic markers based on these variants validated a complete correlation between the GST loss-of-function alleles and white flower in 128 peach accessions. This correlation was further confirmed by silencing of Pp3G013600 using virus-induced gene silencing technology, which reduced anthocyanin accumulation in peach fruit. The new knowledge from this study is useful for designing peach breeding programs to generate cultivars with white flower and fruit skin.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
boymin2015完成签到,获得积分10
刚刚
Nicole完成签到 ,获得积分10
1秒前
乐乐妈完成签到,获得积分10
1秒前
Yxy2021完成签到,获得积分10
1秒前
SONG发布了新的文献求助30
2秒前
研友_Zb1rln完成签到,获得积分10
3秒前
LZL完成签到 ,获得积分10
3秒前
迷人的寒风完成签到,获得积分10
3秒前
xiaowang完成签到,获得积分10
3秒前
3秒前
fenghp发布了新的文献求助10
4秒前
研友_VZG7GZ应助杰尼龟采纳,获得10
4秒前
黄黄完成签到,获得积分10
4秒前
5秒前
5秒前
宋晓静完成签到,获得积分10
5秒前
顺利的乌冬面完成签到,获得积分10
6秒前
赵芳完成签到,获得积分10
6秒前
cfplhys完成签到,获得积分10
6秒前
6秒前
iFaceDOG发布了新的文献求助10
7秒前
芝麻福福完成签到,获得积分10
7秒前
李故完成签到 ,获得积分10
8秒前
研友_nqv2WZ完成签到,获得积分10
8秒前
江小鱼在查文献完成签到,获得积分10
8秒前
8秒前
tender完成签到,获得积分10
9秒前
坐忘道完成签到 ,获得积分10
9秒前
贪玩鸵鸟完成签到,获得积分10
9秒前
喵喵完成签到,获得积分10
9秒前
悉达多发布了新的文献求助10
9秒前
思源应助zed66采纳,获得10
10秒前
包容的若风完成签到 ,获得积分10
10秒前
vffg完成签到,获得积分10
10秒前
微微发布了新的文献求助10
10秒前
俭朴从安完成签到,获得积分10
10秒前
10秒前
道阻且长完成签到,获得积分10
10秒前
火星上的铃铛完成签到,获得积分10
11秒前
五五完成签到,获得积分10
11秒前
高分求助中
Nickel, Cobalt and Palladium Catalysed Infarction with Ventricular following rich structural diversity 1000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968608
求助须知:如何正确求助?哪些是违规求助? 3513486
关于积分的说明 11168243
捐赠科研通 3248926
什么是DOI,文献DOI怎么找? 1794540
邀请新用户注册赠送积分活动 875188
科研通“疑难数据库(出版商)”最低求助积分说明 804676