色谱法
瑞戈非尼
剂型
化学
乙腈
三氟乙酸
高效液相色谱法
稀释剂
保留时间
相(物质)
分析化学(期刊)
医学
核化学
有机化学
内科学
癌症
结直肠癌
作者
R. Raut,Abhijeet D. Patil,Vijaya Kumar Munipalli,Mrunali R. Patel,R. M. Singh,B Fegade,V. H. Bhaskar
出处
期刊:Indian drugs
[Indian Drug Manufacturers' Association (IDMA)]
日期:2018-06-28
卷期号:55 (06): 63-68
被引量:1
标识
DOI:10.53879/id.55.06.11047
摘要
A simple precise and rapid Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method has been developed for quantitative determination of Regorafenib in tablet dosage form. In this method Hypersil Gold (C18, 150mm× 4.6mm id, 3μ) column with mobile phase consisting of Trifluoroacetic acid (0.2% v/v) and Acetonitrile in the ratio of (50: 50 v/v) at 400C in an isocratic mode was used. The detection was carried out at 260 nm and 20μL injection volume was selected with the flow rate 1mL/min. The linearity range of Regorafenib shows concentration between 5-200 μg/mL. The regression coefficient obtained was 0.999. Retention time of Regorafenib was found to be 6.49 minutes. Acetonitrile and Water in the ratio of (3:1) was used as a diluent. The method was validated as per ICH guidelines and is simple, fast, accurate, precise and can be applied for routine quality control analysis of Regorafenib in tablet dosage form.
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