Ubiquitylation of lipopolysaccharide by RNF213 during bacterial infection

泛素 泛素连接酶 细胞生物学 胞浆 脂多糖 生物 生物化学 化学 免疫学 基因
作者
Elsje G. Otten,Emma Werner,Ana Crespillo-Casado,Keith B. Boyle,Vimisha Dharamdasani,Claudio Pathe,Balaji Santhanam,Felix Randow
出处
期刊:Nature [Springer Nature]
卷期号:594 (7861): 111-116 被引量:265
标识
DOI:10.1038/s41586-021-03566-4
摘要

Ubiquitylation is a widespread post-translational protein modification in eukaryotes and marks bacteria that invade the cytosol as cargo for antibacterial autophagy1–3. The identity of the ubiquitylated substrate on bacteria is unknown. Here we show that the ubiquitin coat on Salmonella that invade the cytosol is formed through the ubiquitylation of a non-proteinaceous substrate, the lipid A moiety of bacterial lipopolysaccharide (LPS), by the E3 ubiquitin ligase ring finger protein 213 (RNF213). RNF213 is a risk factor for moyamoya disease4,5, which is a progressive stenosis of the supraclinoid internal carotid artery that causes stroke (especially in children)6,7. RNF213 restricts the proliferation of cytosolic Salmonella and is essential for the generation of the bacterial ubiquitin coat, both directly (through the ubiquitylation of LPS) and indirectly (through the recruitment of LUBAC, which is a downstream E3 ligase that adds M1-linked ubiquitin chains onto pre-existing ubiquitin coats8). In cells that lack RNF213, bacteria do not attract ubiquitin-dependent autophagy receptors or induce antibacterial autophagy. The ubiquitylation of LPS on Salmonella that invade the cytosol requires the dynein-like core of RNF213, but not its RING domain. Instead, ubiquitylation of LPS relies on an RZ finger in the E3 shell. We conclude that ubiquitylation extends beyond protein substrates and that ubiquitylation of LPS triggers cell-autonomous immunity, and we postulate that non-proteinaceous substances other than LPS may also become ubiquitylated. Upon Salmonella invasion of the mammalian cytosol, ubiquitylation of a non-proteinaceous substrate—the lipid A moiety of bacterial lipopolysaccharide—by the E3 ubiquitin ligase RNF213 marks the bacteria as cargo for antibacterial autophagy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小鸭子发布了新的文献求助30
1秒前
1秒前
唠叨的黄蜂完成签到,获得积分10
1秒前
鲸鱼完成签到,获得积分10
1秒前
珊珊完成签到,获得积分10
1秒前
1秒前
hfy发布了新的文献求助10
2秒前
GJH完成签到,获得积分10
2秒前
万能图书馆应助安静的臻采纳,获得10
2秒前
发文章完成签到,获得积分10
2秒前
3秒前
zheng能量发布了新的文献求助10
5秒前
搜集达人应助科研通管家采纳,获得10
6秒前
6秒前
hadfunsix完成签到 ,获得积分10
6秒前
小马甲应助科研通管家采纳,获得10
6秒前
6秒前
7秒前
流水完成签到,获得积分10
7秒前
8秒前
西三环北路第一深情完成签到,获得积分10
8秒前
共享精神应助mmm采纳,获得10
9秒前
阔达初南完成签到 ,获得积分10
9秒前
9秒前
yang完成签到,获得积分10
10秒前
不搭完成签到,获得积分10
10秒前
czb666完成签到,获得积分20
10秒前
10秒前
赘婿应助lucifer0922采纳,获得20
10秒前
11秒前
hh哈哈完成签到,获得积分10
11秒前
科目三应助swordlee采纳,获得10
11秒前
黄小佳发布了新的文献求助10
12秒前
丘比特应助baby采纳,获得10
12秒前
12秒前
13秒前
柚子皮完成签到,获得积分10
13秒前
KYSLCc发布了新的文献求助10
14秒前
Sunny发布了新的文献求助30
14秒前
Akim应助真实的青旋采纳,获得50
15秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Kelsen’s Legacy: Legal Normativity, International Law and Democracy 1000
Handbook on Inequality and Social Capital 800
Conference Record, IAS Annual Meeting 1977 610
Interest Rate Modeling. Volume 3: Products and Risk Management 600
Interest Rate Modeling. Volume 2: Term Structure Models 600
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3546676
求助须知:如何正确求助?哪些是违规求助? 3123726
关于积分的说明 9356475
捐赠科研通 2822353
什么是DOI,文献DOI怎么找? 1551369
邀请新用户注册赠送积分活动 723332
科研通“疑难数据库(出版商)”最低求助积分说明 713721