水解物
化学
抗原性
水解
低过敏性
色谱法
酶水解
固定化酶
酶
酪蛋白
食品科学
生物化学
生物
过敏
抗原
过敏原
免疫学
遗传学
作者
Hongbo Li,Jinghua Yu,Airong Qin,Feifei Yang,Dingkuo Liu,Hongjuan Li,Jinghua Yu
标识
DOI:10.1111/1750-3841.16189
摘要
Abstract Enzymatic hydrolysis is the most commonly used method to reduce the antigenicity of milk protein, but free protease is unstable and difficult to recycle after application. In this study, alcalase and neutrase were selected for immobilization on the modified magnetic Fe 3 O 4 nanoparticles. The reusability of the immobilized enzyme was 68.23% of the total starting activity after 5 recycling batches. The optimal hydrolysis conditions were an enzyme to substrate ratio of 6000 U/g and reaction at 50℃ and pH 8.5 for 3 h. Under these conditions, 22.76% hydrolysis of hydrolysate was achieved, and the antigenicity reduction rates of β‐lactoglobulin and casein were 21.34% and 30.89%, respectively. In addition, 82.75% of the hydrolysate had a molecular weight less than 1 kDa, and free amino acids represented 13.65% of the sample. This result showed that the hydrolysis with immobilized enzyme was similar to that with free enzyme and the immobilized enzyme could be applied to produce hypoallergenic hydrolysate. Practical Application Reduces milk protein allergenicity.
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