单元格排序
原生质体
拟南芥
植物细胞
拟南芥
清脆的
转化(遗传学)
生物
突变
绿色荧光蛋白
细胞生物学
分类
细胞
基因
悬浮培养
计算生物学
分子生物学
植物
细胞培养
遗传学
突变
突变体
计算机科学
程序设计语言
作者
Ward Decaestecker,Norbert Bollier,Rafael Andrade Buono,Moritz K. Nowack,Thomas B. Jacobs
标识
DOI:10.1007/978-1-0716-2164-6_15
摘要
Fluorescence-activated cell sorting (FACS) allows for the enrichment of specific plant cell populations after protoplasting. In this book chapter, we describe the transformation and protoplasting of an Arabidopsis thaliana cell suspension culture (PSB-D, derived from MM2d) that can be used for the evaluation of CRISPR vectors in a subpopulation of cells. We also describe the protoplasting of Arabidopsis thaliana cells from the roots and stomatal lineage for the evaluation of tissue-specific gene editing. These protocols allow us to rapidly and accurately quantify various CRISPR systems in plant cells.
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