生物
转基因
非生物胁迫
活性氧
转基因作物
脯氨酸
非生物成分
盐度
转录因子
苹果属植物
细胞生物学
基因
植物
生物化学
生态学
氨基酸
作者
Qinglong Dong,Dingyue Duan,Jiale He,Wenqian Zheng,Dong Huang,Qian Wang,Jie Yang,Fengwang Ma,Ke Mao
标识
DOI:10.1016/j.scienta.2022.111077
摘要
Soil salinity is a major environmental threat to plant growth, development, and yield. Valine-glutamine (VQ) motif-containing proteins (VQ proteins) are involved in plant growth, development, and responses to abiotic and biotic stressors. However, the relationship between salt stress and VQ proteins has not been studied in horticultural crops. In this study, overexpression of the apple VQ family gene MdVQ37 markedly reduced the salt stress tolerance of transgenic apple plants. Physiological and biochemical assays suggested that the growth limitation, reactive oxygen species production, reduced photosynthetic capacity, and imbalance of ion homeostasis caused by salt stress were aggravated in transgenic lines compared with wild-type (WT) plants. In addition, compared with WT plants, MdVQ37-overexpressing transgenic apple lines showed significantly lower transcript levels of genes associated with ion homeostasis and encoding stress-related transcription factors under salt stress. In summary, we propose that MdVQ37 plays a negative role in the salt stress response of apple, primarily by affecting reactive oxygen species elimination, ion homeostasis, and expression levels of stress-related transcription factors.
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