一氧化氮
一氧化氮合酶
发病机制
葡萄膜炎
亚硝酸盐
化学
炎症
睫状体
药理学
免疫学
医学
病理
硝酸盐
有机化学
作者
Olivier Goureau,J. L. Bellot,B. Thillaye,Yves Courtois,Y. de Kozak
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1995-06-15
卷期号:154 (12): 6518-6523
被引量:125
标识
DOI:10.4049/jimmunol.154.12.6518
摘要
Abstract Nitric oxide (NO) has been implicated in the pathogenesis of several inflammatory diseases. In the present study, we investigated the potential role of NO in an ocular model of inflammation, endotoxin-induced uveitis (EIU), in Lewis rats. Injection of LPS in one footpad induces severe uveitis after 16 h, which is accompanied by an increase of NO in the aqueous and vitreous humors, as evaluated by nitrite assay. Reverse transcriptase-PCR experiments reveal a large increase of inducible NO synthase (iNOS) mRNA in the iris/ciliary body, from 2 to 24 h after LPS treatment. In the retina, maximal increase of iNOS mRNA was detected 16 h after LPS treatment. Two i.p. injections of the NOS inhibitor, NG-nitro-L-arginine methyl ester (L-NAME), which inhibits nitrite release in the aqueous and vitreous humors, profoundly reduce clinical and histologic inflammation in EIU rats. These results implicate the NO pathway in the pathogenesis of EIU and demonstrate the possibility of modulating this inflammatory disease by injection of a NOS inhibitor.
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