重组酶聚合酶扩增
奇异变形杆菌
微生物学
粪肠球菌
肺炎克雷伯菌
生物
聚合酶链反应
铜绿假单胞菌
微生物培养
肠球菌
泌尿系统
细菌
大肠杆菌
病毒学
抗生素
金黄色葡萄球菌
基因
遗传学
内分泌学
作者
Balakrishnan Raja,Heather J. Goux,Archana Marapadaga,S. Rajagopalan,Katerina Kourentzi,Richard C. Willson
摘要
To develop and evaluate the performance of a panel of isothermal real-time recombinase polymerase amplification (RPA) assays for detection of common bacterial urinary tract infection (UTI) pathogens.The panel included RPAs for Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterococcus faecalis. All five RPAs required reaction times of under 12 min to reach their lower limit of detection of 100 genomes per reaction or less, and did not cross-react with high concentrations of nontarget bacterial genomic DNA. In a 50-sample retrospective clinical study, the five-RPA assay panel was found to have a specificity of 100% (95% CI, 78-100%) and a sensitivity of 89% (95% CI, 75-96%) for UTI detection.The analytical and clinical validity of RPA for the rapid and sensitive detection of common UTI pathogens was established.Rapid identification of the causative pathogens of UTIs can be valuable in preventing serious complications by helping avoid the empirical treatment necessitated by traditional urine culture's 48-72-h turnaround time. The routine and widespread use of RPA to supplement or replace culture-based methods could profoundly impact UTI management and the emergence of multidrug-resistant pathogens.
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