金黄色葡萄球菌
适体
微生物学
检出限
免疫磁选
溶解
葡萄球菌
色谱法
耐甲氧西林金黄色葡萄球菌
细菌
生物
化学
分子生物学
遗传学
作者
Jinjuan Qiao,Xiangying Meng,Yufang Sun,Qian Li,Ronglan Zhao,Yun Zhang,Jiaqi Wang,Zhengjun Yi
标识
DOI:10.1016/j.mimet.2018.09.011
摘要
Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is of important clinical significance. In this study, a novel aptamer-based fluorometric assay was developed for detection of MRSA in clinical samples by coupling with immunomagnetic separation. The S. aureus cells in clinical specimens were enriched by magnetic separation. Following lysis by staphylococcal lysin, the PBP2a proteins were released from S. aureus cells and detected by the aptamer-based fluorometric assay. Without lengthy period of bacteria cultivation in the traditional susceptibility testing, this test has an overall testing time of only 2 h with the detection limit of 2.63 × 103 and 1.38 × 103 CFU/mL in PBS and spiked nasal swab, respectively. Since it is simple, rapid and sensitive, this method could be used for the detection of MRSA in various clinical samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI