Abstract The nanobiomaterials display unique and distinguished usefulness in day‐to‐day life. The application of nanobiomaterials is being utilized in several segments, including semiconductor industries, cosmetics, and medicines. Alongside their usefulness these nanobiomaterials have potential toxicity which may limit it's further use. As there is sparse information on toxicological facets of nanomaterials available, a proactive approach for evaluation of potential risk of nanobiomaterials should be exercised. This review explains the potential mechanism of nanobiomaterials toxicity and rational approaches for selection of protocols to study toxicity of nanobiomaterials.