清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Transport of Flavanolic Monomers and Procyanidin Dimer A2 across Human Adenocarcinoma Stomach Cells (MKN-28)

化学 碳酸钙-2 原花青素 色谱法 生物利用度 细胞毒性 生物物理学 生物化学 体外 多酚 生物 抗氧化剂 生物信息学
作者
Shuyi Li,Junsheng Li,Yiwei Sun,Yuqi Huang,Jingren He,Zhenzhou Zhu
出处
期刊:Journal of Agricultural and Food Chemistry [American Chemical Society]
卷期号:67 (12): 3354-3362 被引量:10
标识
DOI:10.1021/acs.jafc.9b00378
摘要

It has been proven that A-type procyanidins, containing an additional ether bond, compared to B-type procyanidins are also bioavailable in vitro and in vivo. However, their bioavailability and absorption in the gastrointestinal tract remain uncertain. In this study, a model of the human adenocarcinoma stomach cell line (MKN-28) was established to explore the cellular transport of flavanolic monomers and procyanidin dimer A2, which were isolated from the litchi pericarp extract. After the integrity and permeability of the cell monolayer were ensured by measurement of the transepithelial electrical resistance and the apparent permeability coefficient for Lucifer yellow, the transportation of procyanidins A2 and B2, (−)-epicatechin (EC), and (+)-catechin (CC) was studied at pH 3.0, 5.0, or 7.0 in the apical side, with compound concentrations of 0.05 and 0.1 mg/mL based on the cytotoxicity test. High-performance liquid chromatography and liquid chromatography–mass spectrometry analyses indicated that EC, CC, and A2 were transported in the MKN-28 cell line from 30 to 180 min, while B2 showed no transport. The maximal transport efficiencies of EC, CC, and A2 were 23 ± 0.81, 13.16 ± 1.53, and 16.41 ± 1.36%, respectively, existing at 120, 180, and 120 min of transportation. Laser scanning confocal microscopy analysis presented the dynamic transmission of EC, in accordance with the result of concentration determination, suggesting that the A-type procyanidins are possibly absorbed through the stomach barrier, which is pH- and time-dependent.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
称心如意完成签到 ,获得积分10
9秒前
h41692011完成签到 ,获得积分10
38秒前
dingding发布了新的文献求助10
47秒前
今后应助dingding采纳,获得10
1分钟前
Jj7完成签到,获得积分10
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
彦嘉发布了新的文献求助10
1分钟前
斯文败类应助ceeray23采纳,获得20
2分钟前
打打应助Dave采纳,获得10
2分钟前
2分钟前
2分钟前
ceeray23发布了新的文献求助20
2分钟前
sh1ro发布了新的文献求助10
2分钟前
nojego完成签到,获得积分10
3分钟前
脑洞疼应助科研通管家采纳,获得10
3分钟前
顾矜应助科研通管家采纳,获得10
3分钟前
wjx完成签到 ,获得积分10
3分钟前
John完成签到,获得积分10
3分钟前
顾矜应助ceeray23采纳,获得20
3分钟前
4分钟前
Able完成签到,获得积分10
4分钟前
我是笨蛋完成签到 ,获得积分10
4分钟前
ceeray23发布了新的文献求助20
4分钟前
Owen应助董可以采纳,获得10
4分钟前
酷酷妙梦完成签到,获得积分10
4分钟前
科研通AI2S应助彦嘉采纳,获得10
4分钟前
小二郎应助科研通管家采纳,获得10
5分钟前
hhh2018687完成签到,获得积分10
5分钟前
科研通AI2S应助ceeray23采纳,获得20
5分钟前
忘忧Aquarius完成签到,获得积分10
5分钟前
wujiwuhui完成签到 ,获得积分10
5分钟前
Lny发布了新的文献求助30
6分钟前
sh1ro完成签到,获得积分10
6分钟前
luang应助ceeray23采纳,获得40
6分钟前
6分钟前
ww完成签到,获得积分10
6分钟前
斯文败类应助ceeray23采纳,获得20
6分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990550
求助须知:如何正确求助?哪些是违规求助? 3532220
关于积分的说明 11256532
捐赠科研通 3271057
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234