Identification and Peptidomic Profiling of Exosomes in Preterm Human Milk: Insights Into Necrotizing Enterocolitis Prevention

微泡 坏死性小肠结肠炎 母乳 外体 生物 免疫学 医学 内科学 小RNA 遗传学 生物化学 基因
作者
Xingyun Wang,Xiangyun Yan,Le Zhang,Jinyang Cai,Yahui Zhou,Heng Liu,Yin Hu,Wenjuan Chen,Siliang Xu,Peipei Liu,Ting Chen,Jun Zhang,Yan Cao,Zhangbin Yu,Shuping Han
出处
期刊:Molecular Nutrition & Food Research [Wiley]
卷期号:63 (13) 被引量:77
标识
DOI:10.1002/mnfr.201801247
摘要

Human breast milk has been shown to prevent necrotizing enterocolitis (NEC). Although exosomes have been identified in breast milk, their function and components have not been fully addressed. This study is conducted to elucidate the differences in peptidomic complexities between preterm and term milk exosomes.Breast milk samples are collected from healthy lactating mothers who have delivered term and preterm infants. Exosomes are separated and quantified. The protective effects of purified exosomes against NEC are investigated both in vitro and in vivo. The peptidomic complexities in term and preterm milk exosomes are analyzed by iTRAQ LC-MS/MS to screen differentially expressed exosomal peptides. Preterm milk exosomes administration significantly enhances proliferation and migration of intestinal epithelial cells compared with term milk exosomes. A total of 70 peptides are found to be significantly modulated in preterm milk samples compared to term milk samples. Of these, 47 peptides are upregulated, and 23 peptides are downregulated. Bioinformatics analysis suggests several potential regulatory roles of the altered peptides in intestinal epithelial cell function.These results reveal the differences for the first time in peptidomic complexities between preterm and term milk exosomes. Milk exosome administration might be a promising prevention for NEC.
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