Mesoporous materials are materials with high surface area and intrinsic porosity, and therefore have attracted great research interest due to these unique structures. Mesoporous titanium dioxide (TiO₂) is one of the most widely studied mesoporous materials given its special characters and enormous applications. In this article, we highlight the significant work on mesoporous TiO₂ including syntheses and applications, particularly in the field of photocatalysis, energy and biology. Different synthesis methods of mesoporous TiO₂-including sol⁻gel, hydrothermal, solvothermal method, and other template methods-are covered and compared. The applications in photocatalysis, new energy batteries and in biological fields are demonstrated. New research directions and significant challenges of mesoporous TiO₂ are also discussed.