A molecularly imprinted sensing system for specific detection of monosaccharides based on CeO2 hollow nanosphere cascade enzyme system

Glucose oxidase (GOx) has unique catalytic properties and has been widely used in sensing and detection fields. However, the presence of isomers of target molecules greatly affects the catalytic ability and sensing accuracy. Therefore, it is particularly important to improve the specific detection of GOx for monosaccharides. Here, using β-D-glucose as a template molecule, molecularly imprinted polymers (MIPs) are prepared by self-initiated polymerization under mild conditions by continuously generating hydroxyl radicals on the surface of hollow metal cerium oxide (CeO2HNPs). A cascade catalytic system (CeO2[email protected]) with high specificity and selectivity for β-D-glucose detection was obtained. Impressively, the obtained cascade catalytic sensing system (CeO2[email protected]) showed a wide linear range of 1.2 μM–0.8 mM and a low detection limit of 1.0 μM. The isomer experiments show that the sensing system has satisfactory selectivity and excellent recycling stability and long-term shelf stability. Therefore, the synthesized β-D-glucose detection cascade catalytic system has broad application prospects in the precise sensing of monosaccharides and chemical food safety.