适体
双金属片
检出限
电化学
原位
线性范围
吸附
材料科学
化学
纳米技术
电极
化学工程
色谱法
金属
有机化学
物理化学
遗传学
冶金
生物
工程类
作者
Ya Zhang,Ying Xu,Ning Li,Wenhao Ma,Mei Yang,Changjun Hou,Danqun Huo
标识
DOI:10.1016/j.ijhydene.2023.03.169
摘要
Ratiometric electrochemical biosensors have attracted the attention of researchers due to their ability to self-calibrate, which can improve accuracy of the detection. We constructed a radiometric electrochemical aptasensor based on functionalized bimetallic Zn–[email protected] (Fc) and Fe–[email protected] blue (MB) composites with high specific surface area, high electrical conductivity and strong adsorption properties to detect human epidermal growth factor receptor 2 (HER2). In this paper, we synthesized bimetallic Zn–[email protected] by in situ electrochemical synthesis and bimetallic Fe–Co-MOF, and the MB signal was wrapped in Fe–Co-MOF, making it immune to the interference of the external environment. In addition, bimetallic MOFs can also immobilize aptamer on their surfaces through adsorption. Therefore, we used Zn–[email protected]/aptamer as the capture probe for HER2 and Fe–[email protected] as the signal labeling probe to jointly construct the radiometric electrochemical aptasensor. By calculating the ratio of signal peaks, simple, fast and accurate detection of HER2 is achieved. The linear range of the sensor is 0.75–250 pg/mL, and the detection limit is 0.37 pg/mL. It provides a new idea for the detection of breast cancer tumor biomarkers.
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