化学
核酸扩增试验
检出限
病毒
核酸
甲型流感病毒
逆转录环介导等温扩增
病菌
病毒学
核糖核酸
逆转录酶
色谱法
微生物学
基因
生物化学
沙眼衣原体
生物
作者
Yidan Zhu,Xijuan Gu,Qu Tang,Wenjun Jiang,Rui Xia,Jing Zhang,Haiwei Ji,Yuling Qin,Li Wu
标识
DOI:10.1021/acs.analchem.4c01570
摘要
The H1N1 influenza virus is a significant pathogen responsible for seasonal influenza, and its frequent outbreaks pose substantial challenges to global public health. The present study successfully developed a lateral flow analysis platform that integrates reverse transcription-free exponential amplification reaction (RTF-EXPAR) and hybridization chain reaction (HCR) processes with functionalized quantum dots for the direct detection of H1N1 influenza virus RNA, eliminating the need for reverse transcription. The fluorescence signal on the band recorded with a smartphone can be utilized for the quantitative determination of the target. Interestingly, the dual signal amplification strategy exhibits high sensitivity with a remarkably low detection limit of 10 aM. Moreover, this platform exhibits excellent flexibility and universality, where the various pathogens can be determined by replacing the specific nucleic acid fragments in RTF-EXPAR. The aforementioned advantages reveal its huge potential in the early diagnosis of H1N1 influenza virus infection and developing point-of-care testing (POCT) equipment for nucleic acid analysis.
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