化学
病毒
甲型流感病毒
H1N1流感
流量(数学)
病毒学
色谱法
2019年冠状病毒病(COVID-19)
机械
内科学
医学
生物
物理
传染病(医学专业)
疾病
作者
Yong Zhu,Xijuan Gu,Qu Tang,Wenjun Jiang,Rui Xia,Jing Zhang,Haiwei Ji,Yuling Qin,Li Wu
标识
DOI:10.1021/acs.analchem.4c01570
摘要
The H1N1 influenza virus is a significant pathogen responsible for seasonal influenza, and its frequent outbreaks pose substantial challenges to global public health. The present study successfully developed a lateral flow analysis platform that integrates reverse transcription-free exponential amplification reaction (RTF-EXPAR) and hybridization chain reaction (HCR) processes with functionalized quantum dots for the direct detection of H1N1 influenza virus RNA, eliminating the need for reverse transcription. The fluorescence signal on the band recorded with a smartphone can be utilized for the quantitative determination of the target. Interestingly, the dual signal amplification strategy exhibits high sensitivity with a remarkably low detection limit of 10 aM. Moreover, this platform exhibits excellent flexibility and universality, where the various pathogens can be determined by replacing the specific nucleic acid fragments in RTF-EXPAR. The aforementioned advantages reveal its huge potential in the early diagnosis of H1N1 influenza virus infection and developing point-of-care testing (POCT) equipment for nucleic acid analysis.
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