光形态发生
拟南芥
水稻
拟南芥
细胞生物学
功能分歧
植物
适应
光防护
转基因水稻
核定位序列
遗传学
核心
基因
生物
转基因
突变体
基因家族
基因表达
光合作用
转基因作物
作者
Hu Shan,Yihan Chen,Chongzhen Qian,Hui Ren,Xinwen Liang,Wenjing Tao,Yanling Chen,Jue Wang,Yuan Dong,Jiupan Han,Xinhao Ouyang,Xi Huang
标识
DOI:10.1038/s41467-024-50755-6
摘要
In plants, the conserved plant-specific photoreceptor UV RESISTANCE LOCUS 8 (UVR8) perceives ultraviolet-B (UV-B) light and mediates UV-B-induced photomorphogenesis and stress acclimation. In this study, we reveal that UV-B light treatment shortens seedlings, increases stem thickness, and enhances UV-B stress tolerance in rice (Oryza sativa) via its two UV-B photoreceptors OsUVR8a and OsUVR8b. Although the rice and Arabidopsis (Arabidopsis thaliana) UVR8 (AtUVR8) photoreceptors all form monomers in response to UV-B light, OsUVR8a, and OsUVR8b function is only partially conserved with respect to AtUVR8 in UV-B-induced photomorphogenesis and stress acclimation. UV-B light and CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) promote the nuclear accumulation of AtUVR8; by contrast, OsUVR8a and OsUVR8b constitutively localize to the nucleus via their own nuclear localization signals, independently of UV-B light and the RING-finger mutation of OsCOP1. We show that OsCOP1 negatively regulates UV-B responses, and shows weak interaction with OsUVR8s, which is ascribed to the N terminus of OsCOP1, which is conserved in several monocots. Furthermore, transcriptome analysis demonstrates that UV-B-responsive gene expression differs globally between Arabidopsis and rice, illuminating the evolutionary divergence of UV-B light signaling pathways between monocot and dicot plants. Differently from Arabidopsis, rice UV-B photoreceptors show a non-classical localization mechanism and protein interaction mode to mediate UV-B responses, suggestive of evolutionary divergence of UV-B light signaling between monocot and dicot plants.
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