颅骨
淀粉样蛋白(真菌学)
荧光
化学
生物物理学
纤维
病理
荧光寿命成像显微镜
红外线的
淀粉样纤维
淀粉样β
解剖
生物
医学
生物化学
光学
疾病
物理
作者
Steven S. Hou,Joyce Yang,Jeong Heon Lee,Yeseo Kwon,María Calvo-Rodríguez,Kai Bao,Sung Ahn,Satoshi Kashiwagi,Anand T. N. Kumar,Brian J. Bacskai,Hak Soo Choi
标识
DOI:10.1038/s41551-023-01003-7
摘要
Non-invasive methods for the in vivo detection of hallmarks of Alzheimer's disease can facilitate the study of the progression of the disease in mouse models and may enable its earlier diagnosis in humans. Here we show that the zwitterionic heptamethine fluorophore ZW800-1C, which has peak excitation and emission wavelengths in the near-infrared optical window, binds in vivo and at high contrast to amyloid-β deposits and to neurofibrillary tangles, and allows for the microscopic imaging of amyloid-β and tau aggregates through the intact skull of mice. In transgenic mouse models of Alzheimer's disease, we compare the performance of ZW800-1C with that of the two spectrally similar heptamethine fluorophores ZW800-1A and indocyanine green, and show that ZW800-1C undergoes a longer fluorescence-lifetime shift when bound to amyloid-β and tau aggregates than when circulating in blood vessels. ZW800-1C may prove advantageous for tracking the proteinic aggregates in rodent models of amyloid-β and tau pathologies. A fluorophore with peak excitation and emission wavelengths in the near-infrared window allows for the microscopic imaging of amyloid-β and tau aggregates through the intact skull in mouse models of Alzheimer's disease.
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