Prophylactic effect of pectic oligosaccharides against poly I: C‐ induced virus‐like infection in BALB/c mice

Journal of Food BiochemistryVolume 46, Issue 12 e14459 ORIGINAL ARTICLE Prophylactic effect of pectic oligosaccharides against poly I: C- induced virus-like infection in BALB/c mice Nidhi Sori, Nidhi Sori Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, IndiaSearch for more papers by this authorSaarika Pothuvan Kunnummal, Saarika Pothuvan Kunnummal Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, India CSIR-Academy of Scientific & Innovative Research, Ghaziabad, IndiaSearch for more papers by this authorMuthuKumar Serva Peddha, MuthuKumar Serva Peddha orcid.org/0000-0002-0010-4219 CSIR-Academy of Scientific & Innovative Research, Ghaziabad, India Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysuru, IndiaSearch for more papers by this authorMahejibin Khan, Corresponding Author Mahejibin Khan mahejibin@cftri.res.in orcid.org/0000-0001-5331-8643 Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, India CSIR-Academy of Scientific & Innovative Research, Ghaziabad, India Correspondence Mahejibin Khan, Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, Karnataka 570020, India. Email: mahejibin@cftri.res.inSearch for more papers by this author Nidhi Sori, Nidhi Sori Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, IndiaSearch for more papers by this authorSaarika Pothuvan Kunnummal, Saarika Pothuvan Kunnummal Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, India CSIR-Academy of Scientific & Innovative Research, Ghaziabad, IndiaSearch for more papers by this authorMuthuKumar Serva Peddha, MuthuKumar Serva Peddha orcid.org/0000-0002-0010-4219 CSIR-Academy of Scientific & Innovative Research, Ghaziabad, India Department of Biochemistry, CSIR - Central Food Technological Research Institute, Mysuru, IndiaSearch for more papers by this authorMahejibin Khan, Corresponding Author Mahejibin Khan mahejibin@cftri.res.in orcid.org/0000-0001-5331-8643 Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, India CSIR-Academy of Scientific & Innovative Research, Ghaziabad, India Correspondence Mahejibin Khan, Department of Microbiology and Fermentation Technology, CSIR - Central Food Technological Research Institute, Mysuru, Karnataka 570020, India. Email: mahejibin@cftri.res.inSearch for more papers by this author First published: 14 October 2022 https://doi.org/10.1111/jfbc.14459Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Pectin oligosaccharides (POS) are pectin-derived prebiotics that exerts anti-inflammatory effects on the host and stimulates an innate immune response. The role of POS in protective immunity against viral infections is not very obvious. Therefore, the prophylactic effect of POS in the mouse model induced by Poly I: C mimicking viral infection was examined. Mice fed POS showed a significant (p ≤ .05) increase in IgG, sIgA, IgA, IL-12, and a significant (p ≤ .05) decrease in the concentration of pro-inflammatory cytokines IL-5, IL-6, IL-13 and IL-17 in lung and blood serum after Poly I: C stimulation. However, the control group could not inhibit pro-inflammatory cytokines. POS also promoted the growth of the Lactobacillus, Prevotella, Rilenellaceae, and Lachanospiraceae groups. Therefore, this study demonstrate that POS has the potiential to protect against viral inflammation by altering gut microbiota and activating mucosal immunity. Practical applications POS is 2–10 mer oligomers of pectin. The human gastrointestinal tract lacks the enzyme to break down POS. They are fermented by gut bacteria in the colon and stimulate the proliferation of specific gut bacteria that are positively correlated with the production of anti-inflammatory cytokines and SCFA. POS also stimulates the secretion of IgA, which inhibits bacterial and viral adhesion and protects the host. Therefore, POS can be used as a functional food ingredient in food to stimulate a specific group of gut bacteria and enhance preventive immunity. CONFLICT OF INTEREST The authors declared that they have no conflict of interest. Open Research DATA AVAILABILITY STATEMENT NSG data has been submitted at NCBI under bioproject no. PRJNA731640. Other details are available from the authors. Volume46, Issue12December 2022e14459 RelatedInformation