Environmental DNA metabarcoding effectively monitors terrestrial species by using urban green spaces

环境DNA 地理 生态学 环境科学 生物多样性 生物
作者
Whee-Moon Kim,Hyunjung Lee,Wonkyong Song
出处
期刊:Urban Forestry & Urban Greening [Elsevier BV]
卷期号:78: 127782-127782 被引量:2
标识
DOI:10.1016/j.ufug.2022.127782
摘要

Ecological corridors and urban parks are created to connect habitats that become isolated due to urban development and integrate ecological, social, and cultural functions. Establishing and implementing the ecological function of urban green structures and conservation plans for urban terrestrial species requires prioritizing developing an understanding of the community structure of urban terrestrial species. However, understanding the appearance and distribution of terrestrial species in urban ecosystems is often neglected or lacking; furthermore, the inaccurate detection of species distribution can create biased estimates of species occupancy. Recently, an applied research method for detecting terrestrial species such as mammals and birds has been proposed based on molecular biology such as environmental DNA (eDNA) metabarcoding. We installed artificial water tanks (mesocosm) in urban green spaces (Urban forests; UFs, Urban ecological corridors; UECs, Urban parks; UPs), applying eDNA metabarcoding as a tool to detect wild terrestrial species, and camera traps (CTs) and field surveys were performed in parallel to verify the results. We detected 22 urban terrestrial species only by eDNA metabarcoding and identified 43 species in total by performing eDNA metabarcoding, CTs, and field surveys in parallel. In addition, we identified 31 species of UFs, 13 species of UECs, and 28 species of UPs of urban terrestrial species using the three species survey methods in parallel. We confirmed that eDNA metabarcoding is very useful for detecting small species that are difficult to detect with the naked eye and internal species vulnerable to disturbance. However, since some species were only detected by CTs or field surveys due to DNA falsenegative errors or the species’ life-history characteristics, it was judged that the species inventory can be effectively collected using several monitoring techniques. • We successfully monitored most of the present terrestrial species using urban green spaces in just seven days, complementing environmental DNA metabarcoding, camera traps, and fieldwork. • In an urban ecosystem, eDNA metabarcoding was performed, in spaces where it was difficult to collect river samples due to large disturbances, with an artificial water tank containing distilled water, and clear spatiotemporal information of the recorded species was provided. • The sensitivity of eDNA metabarcoding was affected depending on the degree of human disturbance. In particular, in the case of terrestrial species within the city, the field survey was still a stronger research technique than eDNA and camera traps. • Urban ecological corridors and urban parks negatively impacted the inter-forest movement and habitation of terrestrial species due to the use of urban residents such as trails, but the use of species was confirmed as much as urban forests; in particular, mammals shared a home-range by dividing day/night time zones with urban residents due to their nocturnal nature. • The introduction of eDNA metabarcoding and camera traps in urban ecosystems can fill gaps in understanding and knowledge about changes in urban biodiversity by inducing the participation of citizens interested in ecology.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
大方弘文发布了新的文献求助10
刚刚
YooM发布了新的文献求助10
刚刚
1秒前
风趣的惜天完成签到 ,获得积分10
1秒前
冰美式乌龙茶完成签到,获得积分20
2秒前
Ldq发布了新的文献求助10
2秒前
Obsession完成签到 ,获得积分10
3秒前
trans发布了新的文献求助10
6秒前
6秒前
6秒前
大模型应助BakerStreet采纳,获得10
6秒前
8秒前
10秒前
yoyo发布了新的文献求助10
10秒前
彭于彦祖应助杨烔星采纳,获得30
10秒前
一轮明月完成签到 ,获得积分10
11秒前
非鱼鱼完成签到 ,获得积分10
12秒前
万历完成签到,获得积分10
12秒前
朱心怡完成签到,获得积分10
12秒前
小蘑菇应助阳佟听荷采纳,获得10
12秒前
12秒前
独狼完成签到 ,获得积分10
13秒前
13秒前
wxiao发布了新的文献求助10
14秒前
朱晖发布了新的文献求助10
14秒前
14秒前
无糖乐完成签到,获得积分20
15秒前
kma发布了新的文献求助10
17秒前
无糖乐发布了新的文献求助10
17秒前
量子星尘发布了新的文献求助10
18秒前
18秒前
yoyo完成签到,获得积分10
19秒前
亦犹未进完成签到,获得积分10
19秒前
wangxiaobin完成签到 ,获得积分10
20秒前
平淡凡柔发布了新的文献求助10
20秒前
21秒前
wxiao完成签到,获得积分10
22秒前
CHEN__02_发布了新的文献求助10
22秒前
英姑应助无糖乐采纳,获得10
23秒前
学习使我快乐1917完成签到,获得积分10
24秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969628
求助须知:如何正确求助?哪些是违规求助? 3514448
关于积分的说明 11174217
捐赠科研通 3249822
什么是DOI,文献DOI怎么找? 1795000
邀请新用户注册赠送积分活动 875537
科研通“疑难数据库(出版商)”最低求助积分说明 804856