多糖
食品科学
发酵
萃取(化学)
瘤胃球菌
DPPH
化学
阿布茨
肠道菌群
热水抽汽
拟杆菌
抗氧化剂
单糖
益生元
生物化学
生物
色谱法
微生物学
细菌
遗传学
作者
Yi Liang,Wanguo Yu,Huatian Wang,Lingyun Yao,Zeng‐Yang He,Min Sun,Tao Feng,Chuang Yu,Heng Yue
标识
DOI:10.1016/j.ijbiomac.2024.130174
摘要
In this study, flash extraction was used to rapidly extract water-soluble polysaccharides from Ulva linza. The optimal extraction process for the flash extraction was determined by Box–Behnken design with extraction temperature 80 °C, extraction time 117 s, liquid-solid ratio 46:1 (mL/g) and a corresponding yield of 18.5 %. The crude Ulva linza polysaccharides (CULP) were subsequently isolated by chromatography technology to obtain purified Ulva linza polysaccharide (ULP) and characterized by monosaccharide composition and molecular weight determination analysis. Furthermore, the antioxidant bioactivity of ULP was studied and the results revealed that it had a good scavenging effect on DPPH, ABTS and OH, with IC50 values of 149.2 μg/mL, 252.5 μg/mL and 1073 μg/mL, respectively. After in vitro fermentation by human fecal microbiota, the pH value of fermentation culture significantly decreased to 5.06, suggesting that ULP could be hydrolyzed and utilized by gut microbiota. The abundance of beneficial bacteria including Bacteroides, Parabacteroides and Faecalibacterium was improved. Meanwhile, the relative abundance of Prevotella, Blautia and Ruminococcus was decreased, and the low ratio of these organisms might reveal positive effects on maintaining the balance of gut microbial biodiversity. These results suggested that the composition of the human gut microbiota could be modulated by ULP, and ULP might possess the potential to maintain gut homeostasis and improve human intestinal health.
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