先天免疫系统
肿瘤微环境
癌症研究
生物
下调和上调
DNA损伤
免疫系统
白细胞介素8
炎症
免疫学
细胞生物学
基因
DNA
遗传学
作者
Faya Zhang,Oscar Mulvaney,Enrique Salcedo,Subrata Manna,James Z. Zhu,Tao Wang,Chul Ahn,Laurentiu M. Pop,Raquibul Hannan
出处
期刊:Cancers
[MDPI AG]
日期:2023-12-01
卷期号:15 (23): 5686-5686
被引量:1
标识
DOI:10.3390/cancers15235686
摘要
The early events that lead to the inflammatory and immune-modulatory effects of radiation therapy (RT) in the tumor microenvironment (TME) after its DNA damage response activating the innate DNA-sensing pathways are largely unknown. Neutrophilic infiltration into the TME in response to RT is an early innate inflammatory response that occurs within 24–48 h. Using two different syngeneic murine tumor models (RM-9 and MC-38), we demonstrated that CXCR2 blockade significantly reduced RT-induced neutrophilic infiltration. CXCR2 blockade showed the same effects on RT-induced tumor inhibition and host survival as direct neutrophil depletion. Neutrophils highly and preferentially expressed CXCR2 compared to other immune cells. Importantly, RT induced both gene and protein expression of CXCLs in the TME within 24 h, attracting neutrophils into the tumor. Expectedly, RT also upregulated the gene expression of both cGAS and AIM2 DNA-sensing pathways in cGAS-positive MC-38 tumors but not in cGAS-negative RM-9 tumors. Activation of these pathways resulted in increased IL-1β, which is known to activate the CXCLs/CXCR2 axis. Gene ontology analysis of mRNA-Seq supported these findings. Taken together, the findings suggest that the CXCLs/CXCR2 axis mediates the RT-induced innate inflammatory response in the TME, likely translating the effects of innate DNA-sensing pathways that are activated in response to RT-induced DNA damage.
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