作者
Monika Licaj,Rana Mhaidly,Yann Kieffer,Hugo Croizer,Claire Bonneau,Anming Meng,Lounes Djerroudi,Kevin Mujangi-Ebeka,Hocine R. Hocine,Brigitte Bourachot,Ilaria Magagna,Renaud Leclère,Léa Guyonnet,Mylène Bohec,Coralie L. Guerin,Sylvain Baulande,Maud Kamal,Christophe Le Tourneau,Fabrice Lécuru,Véronique Becette,Roman Rouzier,Anne Vincent‐Salomon,Géraldine Gentric,Fatima Mechta‐Grigoriou
摘要
Abstract Although cancer-associated fibroblast (CAF) heterogeneity is well-established, the impact of chemotherapy on CAF populations remains poorly understood. Here we address this question in high-grade serous ovarian cancer (HGSOC), in which we previously identified 4 CAF populations. While the global content in stroma increases in HGSOC after chemotherapy, the proportion of FAP + CAF (also called CAF-S1) decreases. Still, maintenance of high residual CAF-S1 content after chemotherapy is associated with reduced CD8 + T lymphocyte density and poor patient prognosis, emphasizing the importance of CAF-S1 reduction upon treatment. Single cell analysis, spatial transcriptomics and immunohistochemistry reveal that the content in the ECM-producing ANTXR1 + CAF-S1 cluster (ECM-myCAF) is the most affected by chemotherapy. Moreover, functional assays demonstrate that ECM-myCAF isolated from HGSOC reduce CD8 + T-cell cytotoxicity through a Yes Associated Protein 1 (YAP1)-dependent mechanism. Thus, efficient inhibition after treatment of YAP1-signaling pathway in the ECM-myCAF cluster could enhance CD8 + T-cell cytotoxicity. Altogether, these data pave the way for therapy targeting YAP1 in ECM-myCAF in HGSOC.