Chemical Synthesis of Human Milk Oligosaccharides: para‐Lacto‐N‐hexaose and para‐Lacto‐N‐neohexaose

Abstract Human milk oligosaccharides (HMO) have emerged as a very active area of research in glycoscience and nutrition. HMO are involved in the early development of infants and may help to prevent certain diseases. The development of chemical methods for obtaining individual HMO aids the global effort dedicated to understanding the roles of these biomolecules. Reported herein is the chemical synthesis of two common core hexasaccharides found in human milk, i. e. para ‐lacto‐ N ‐hexaose ( p LNH) and para ‐lacto‐ N ‐neohexaose ( p LNnH). After screening multiple leaving groups and temporary protecting group combinations, a 3+3 convergent coupling strategy was found to work best for obtaining these linear glycans.