色谱法
干血
样品制备
化学
样品(材料)
质谱法
采样(信号处理)
滤纸
纤维素
液相色谱-质谱法
干血斑
滤波器(信号处理)
计算机科学
计算机视觉
有机化学
作者
Christina Johannsen,Ago Mrsa,Trine Grønhaug Halvorsen,Léon Reubsaet
标识
DOI:10.1002/jssc.202300394
摘要
This perspective explores the feasibility of smart sampling with dried blood spots for the determination of proteins and peptides from human biomatrices using liquid chromatography coupled to mass spectrometry for clinical purposes. The focus is on innovative approaches to transform filter paper from a mere sample carrier to an active element in sample preparation, with the aim of reducing the need for extensive and intensive sample preparation in the conventional sense. Specifically, we discuss the use of modified cellulose to integrate sample preparation at an early stage of sample handling. The use of paper immobilized with either trypsin or monoclonal antibodies for protein digestion and affinity clean-up is discussed as a potential benefit of starting sample preparation instantly at the moment of sampling to optimize time efficiency and enable faster analysis, diagnosis, and follow-up of patients.
科研通智能强力驱动
Strongly Powered by AbleSci AI